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Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]

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Specification

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Catalog# ET1610-43

Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IF-Cell

  • FC

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Cytokeratin 5 aa 70-110.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, IF-Cell, FC, mIHC

Molecular Weight

Predicted band size: 62 kDa

Positive Control

A431, A431 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human skin tissue, human tonsil tissue, human lung squamous cell carcinoma tissue, human breast carcinoma tissue.

Conjugation

unconjugated

Clone Number

SC62-04

RRID

AB_3069928

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:500-1:2,000

  • IF-Tissue

  • 1:200

  • IF-Cell

  • 1:500

  • FC

  • 1:1,000

  • mIHC

  • 1:1,000

Target

Function

Cytokeratin 5 is commonly found in the cells on the outermost layer of skin in humans and animals. It is encoded by the KRT5 gene and pairs with the type I keratin K14. Cytokeratin 5 has become an important biomarker for different types of cancer, including mesothelioma, breast cancer and lung cancer. It helps differentiate squamous carcinomas from adenocarcinomas. Pathologists use cytokeratin 5 to distinguish mesothelioma from adenocarcinoma, the most common type of lung cancer. Cytokeratin 5/6 cannot identify mesothelioma on its own. Cytokeratin 5/6 is a positive marker for malignant pleural mesothelioma, found in more than three-fourths of cases. It is also found in certain types of lung cancers and breast cancers. Pathologists use cytokeratin 5/6 to stain cancer tissue samples. Cytokeratin 5/6 immunoreactivity is rarely seen in adenocarcinomas of the lung. If a tumor sample shows strong expression of cytokeratin 5/6, it gives pathologists a hint the tumor is malignant mesothelioma rather than a metastatic adenocarcinoma. However, this marker is not effective for all cell types of mesothelioma. Cytokeratin 5/6 staining is usually weak or negative for sarcomatoid mesothelioma, the least common and hardest-to-treat cell type of the asbestos-related cancer. The marker is also not effective in telling the difference between pleural mesothelioma and squamous cell carcinomas. About 25 to 30 percent of all lung cancers are squamous cell carcinomas.

Background References

1. Terada K et al. Clinicopathological features of cytokeratin 5-positive pulmonary adenocarcinoma. Histopathology. 2023 Feb

2. Li A et al. Altered cytokeratin 5 expression in breast lobular myoepithelial cells. J Clin Pathol. 2024 Jul

Sequence Similarity

Belongs to the intermediate filament family.

Tissue Specificity

Expressed in corneal epithelium (at protein level).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P13647 Human

SwissProt: Q922U2 Mouse

SwissProt: Q6P6Q2 Rat

Synonyms

58 kDa cytokeratin antibody

CK-5 antibody

CK5 antibody

Cytokeratin-5 antibody

Cytokeratin5 antibody

DDD antibody

DDD1 antibody

EBS2 antibody

epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody

K2C5_HUMAN antibody

Expand

Images

  • Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/500 dilution and competitor's antibody at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/500 dilution and competitor's antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/500 dilution and competitor's antibody at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/500 dilution and competitor's antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Cytokeratin 5 on different lysates with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/5,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: MDA-MB-468 cell lysate<br />Lane 3: MCF7 cell lysate (negative)<br />Lane 4: Mouse skin tissue lysate<br />Lane 5: Rat skin tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 57 kDa<br /><br />Exposure time: 2 minutes 6 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Cytokeratin 5 on different lysates with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/5,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: MDA-MB-468 cell lysate
    Lane 3: MCF7 cell lysate (negative)
    Lane 4: Mouse skin tissue lysate
    Lane 5: Rat skin tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 62 kDa
    Observed band size: 57 kDa

    Exposure time: 2 minutes 6 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-43) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/800 dilution and competitor's antibody at 1/800 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/800 dilution and competitor's antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/800 dilution and competitor's antibody at 1/800 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43) at 1/800 dilution and competitor's antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma tissue with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,000 dilution.<br /><br />Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with <a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a> for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND&reg; RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma tissue with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/1,000 dilution.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ET1610-43 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/1,500 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 5 (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>) at 1/200 dilution.<br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>, red) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, green) at 1/200 dilution at +4℃ overnight, washed with PBS. iFluorTM 594 conjugate-Goat anti-Rabbit IgG (<a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) and iFluorTM 488 conjugate-Goat anti-Mouse IgG (<a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>)were used as the secondary antibodies at 1/1000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 5 (ET1610-43) at 1/200 dilution and Vimentin (EM0401) at 1/200 dilution.
    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (ET1610-43, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS. iFluorTM 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluorTM 488 conjugate-Goat anti-Mouse IgG (HA1125)were used as the secondary antibodies at 1/1000 dilution. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>) at 1/200 dilution.<br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>, red) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, green) at 1/200 dilution at +4℃ overnight, washed with PBS. iFluorTM 594 conjugate-Goat anti-Rabbit IgG (<a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) and iFluorTM 488 conjugate-Goat anti-Mouse IgG (<a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>)were used as the secondary antibodies at 1/1000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (ET1610-43) at 1/200 dilution and Vimentin (EM0401) at 1/200 dilution.
    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (ET1610-43, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS. iFluorTM 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluorTM 488 conjugate-Goat anti-Mouse IgG (HA1125)were used as the secondary antibodies at 1/1000 dilution. DAPI was used as nuclear counterstain.

  • Flow cytometric analysis of A431 cells labeling Cytokeratin 5.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling Cytokeratin 5.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1610-43, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cytokeratin 5 on different lysates with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/5,000 dilution.<br /><br />Lane 1: A431-si NT cell lysate<br />Lane 2: A431-si Cytokeratin 5 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 57 kDa<br /><br />Exposure time: 5 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cytokeratin 5 on different lysates with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/5,000 dilution.

    Lane 1: A431-si NT cell lysate
    Lane 2: A431-si Cytokeratin 5 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 62 kDa
    Observed band size: 57 kDa

    Exposure time: 5 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-43) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,000 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/1,000 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 5 antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,000 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-43" style="font-weight: bold;text-decoration: underline;">ET1610-43</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 5 antibody (ET1610-43) at 1/1,000 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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