DC-SIGN Rabbit Polyclonal Antibody
Catalog# ER1907-23
DC-SIGN Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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FC
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Human
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unconjugated
Overview
Product Name
DC-SIGN Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human DC-SIGN/CD209 51-150/1404
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue, FC
Molecular Weight
45 KDa
Positive Control
Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
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FC
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1μg/Test
Target
Function
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
Background References
暂无
Subcellular Location
Isoform 1, 2, 3, 4, 5, : Cell membrane; Single-pass type II membrane protein (Probable). Isoform 6, 7, 8, 9, 10, 11, 12: Secreted (Probable).
UNIPROT
Synonyms
C type lectin domain family 4 member L antibody
C-type lectin domain family 4 member L antibody
CD 209 antibody
CD209 antibody
CD209 antigen antibody
CD209 antigen-like protein A antibody
CD209 molecule antibody
CD209_HUMAN antibody
Cd209a antibody
CDSIGN antibody
ExpandImages
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Sample:
Hela Cell Lysate at 40 ug
Hcclm3 Cell Lysate at 40 ug
Primary: Anti-DC-SIGN (ER1907-23) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD -
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (ER1907-23) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (ER1907-23) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-DC-SIGN antibody（ER1907-23）
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC.
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
DC-SIGN Recombinant Rabbit Monoclonal Antibody [JE31-24]
Application: WB
Reactivity: Human
Conjugate: unconjugated
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