DDX58 Recombinant Rabbit Monoclonal Antibody [JE60-05]
Catalog# HA720090
DDX58 Recombinant Rabbit Monoclonal Antibody [JE60-05]
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WB
-
Human
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unconjugated
Overview
Product Name
DDX58 Recombinant Rabbit Monoclonal Antibody [JE60-05]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human DDX58 aa 250-450/925.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 107 kDa
Positive Control
HUVEC cell lysates.
Conjugation
unconjugated
Clone Number
JE60-05
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and proinflammatory cytokines. Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments. The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms. Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons.
Background References
1. Xian H. et. al. LRRC59 modulates type I interferon signaling by restraining the SQSTM1/p62-mediated autophagic degradation of pattern recognition receptor DDX58/RIG-I. Autophagy. 2020 Mar
2. Ferreira CR. et. al. DDX58 and Classic Singleton-Merten Syndrome. J Clin Immunol. 2019 Jan
Subcellular Location
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Tight junction.
UNIPROT
Synonyms
Ddx58 antibody
DDX58_HUMAN antibody
DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
DEAD (Asp Glu Ala Asp/His) box polypeptide antibody
DEAD box protein 58 antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody
DKFZp434J1111 antibody
DKFZp686N19181 antibody
FLJ13599 antibody
Probable ATP dependent RNA helicase DDX58 antibody
ExpandImages
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Western blot analysis of DDX58 on HUVEC cell lysates with Rabbit anti-DDX58 antibody (HA720090) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720090) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Neuromedin B and its receptor NMBR inhibit H9N2 infection
Journal: Veterinary Microbiology
DOI: 10.1016/j.vetmic.2025.110790
IF: 2.7
Application: WB
Reactivity: Mouse,Human
Publish date: 2025 Nov
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