Skip to content

DNAJC5 Rabbit Polyclonal Antibody

Usd:

Bulk Order Quote | Customization Request

Catalog# ER63138

DNAJC5 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

DNAJC5 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human DNAJC5 aa 151-198.

Species Reactivity

Human, Mouse, Rat, Green monkey, Pig

Validated Applications

WB, IF-Cell, FC(Intra)

Molecular Weight

Predicted band size: 22 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, Hep G2 (Human liver cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse brain (P7) tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

0.2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

  • FC(Intra)

  • 1:200

Target

Function

DnaJ homolog subfamily C member 5, also known as cysteine string protein or CSP is a protein, that in humans encoded by the DNAJC5 gene. It was first described in 1990. In humans, the gene is located on the long arm of chromosome 20 (20q13.33) on the Watson (positive strand). The gene is 40,867 bases in length and the encoded protein has 198 amino acids with a predicted molecular weight of 22.149 kilodaltons (kDa). The weight of the mature protein is 34 kDa. This gene is highly conserved and found both in invertebrates and vertebrates. In humans, a pseudogene of this gene is located on the short arm of chromosome 8.

Background References

1. Lee J et al. Safeguarding Lysosomal Homeostasis by DNAJC5/CSPalpha-Mediated Unconventional Protein Secretion and Endosomal Microautophagy. Front Cell Dev Biol. 2022 May

2. Chen C et al. DNAJC5 facilitates the proliferation and migration of lung adenocarcinoma cells by augmenting EGFR trafficking. Commun Biol. 2025 May

Subcellular Location

Cytoplasm, cytosol, Membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane , Melanosome, Cell membrane.

UNIPROT

SwissProt: Q9H3Z4 Human

SwissProt: P60904 Mouse

SwissProt: P60905 Rat

Synonyms

CLN 4 antibody

CLN4 antibody

CLN4B antibody

CSP antibody

cysteine string protein alpha antibody

Cysteine string protein antibody

DJC5 antibody

DnaJ (Hsp40) homolog subfamily C member 5 antibody

DnaJ homolog subfamily C member 5 antibody

DNAJC 5 antibody

Expand

Images

  • Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (<a href="/products/ER63138" style="font-weight: bold;text-decoration: underline;">ER63138</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate<br />Lane 3: Hep G2 (Human liver cancer cell) cell lysate<br />Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 5: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate<br />Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br />Lane 7: C6 (Rat glioma cell) cell lysate<br />Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER63138" style="font-weight: bold;text-decoration: underline;">ER63138</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 22 kDa<br />Observed band size: 30 kDa

    Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (ER63138) at 1/2,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate
    Lane 3: Hep G2 (Human liver cancer cell) cell lysate
    Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 5: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
    Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
    Lane 7: C6 (Rat glioma cell) cell lysate
    Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER63138, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 22 kDa
    Observed band size: 30 kDa

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Rat <br />Sample: PC-12(Rat pheochromocytoma cell (undifferentiated))<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br /><br />Primary antibody: <a href="/products/ER63138" style="font-weight: bold;text-decoration: underline;">ER63138</a>, 1/100, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature.<br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Rat
    Sample: PC-12(Rat pheochromocytoma cell (undifferentiated))

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: ER63138, 1/100, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (<a href="/products/ER63138" style="font-weight: bold;text-decoration: underline;">ER63138</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain (P7) tissue lysate<br />Lane 2: Mouse kidney tissue lysate<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat kidney tissue lysate<br />Lane 5: Pig brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 14 seconds; ECL: K1801<br /><br />Negative expression of DNAJC5 protein in kidney is consistent with the predicted expression pattern.<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER63138" style="font-weight: bold;text-decoration: underline;">ER63138</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 22 kDa<br />Observed band size: 30 kDa

    ☑ Relative expression (RE)

    Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (ER63138) at 1/2,000 dilution.

    Lane 1: Mouse brain (P7) tissue lysate
    Lane 2: Mouse kidney tissue lysate
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat kidney tissue lysate
    Lane 5: Pig brain tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 14 seconds; ECL: K1801

    Negative expression of DNAJC5 protein in kidney is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER63138, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 22 kDa
    Observed band size: 30 kDa

  • Application: Flow Cytometry (Intra)<br /><br />Species: Rat<br />Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/ER63138" style="font-weight: bold;text-decoration: underline;">ER63138</a>(1/200, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Rat
    Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: ER63138(1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

metafield image

DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38]

Application: WB,IF-Cell,IHC-Fr,IHC-P,FC(Intra),IP

Reactivity: Human,Mouse,Rat,Green monkey,Pig

Conjugate: unconjugated

Products with the same target and pathway

metafield image

DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38] - BSA and Azide free

Application: WB,IF-Cell,IHC-Fr,IHC-P,FC(Intra),IP

Reactivity: Human,Mouse,Rat,Green monkey,Pig

Conjugate: unconjugated

metafield image

DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38]

Application: WB,IF-Cell,IHC-Fr,IHC-P,FC(Intra),IP

Reactivity: Human,Mouse,Rat,Green monkey,Pig

Conjugate: unconjugated