DOCK4 Rabbit Polyclonal Antibody
Catalog# ER1802-17
DOCK4 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
DOCK4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human DOCK4 aa 1,917-1,966 / 1,966.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
225 kDa
Positive Control
HepG2, HUVEC, PC-3M, rat skeletal muscle tissue, human fetal skeletal muscle tissue, human prostate tissue, mouse skeletal muscle tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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WB
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1:500
Target
Function
DOCK 4 (Dedicator of cytokinesis protein 4) is a cytoplasmic peripheral membrane protein that belongs to the DOCK family of cytokinesis-regulating proteins. Expressed ubiquitously with highest expression in prostate, ovary and skeletal muscle, DOCK 4 functions as a guanine nucleotide exchange factor (GEF) that activates the small GTPase Rap 1 and, via this activation, plays a role in the regulation of adherens junctions between cells. Similar to other DOCK family members, DOCK 4 contains an N-terminal SH3 domain, a C-terminal proline-rich region and two internal DOCK homology regions designated DHR1 and DHR2. Defects in the gene encoding DOCK 4 result in the inactivation of Rap 1 and are, thus, implicated in the pathogenesis of various cancers such as ovarian, prostate, glioma and colorectal carcinomas. Four isoforms of DOCK 4 are expressed due to alternative splicing events.
Background References
暂无
Sequence Similarity
Belongs to the DOCK family.
Tissue Specificity
Widely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary.; [Isoform 2]: May be specifically expressed in the brain and eye.
Subcellular Location
Cytosol. Cell membrane.
Synonyms
Dedicator of cytokinesis protein 4 antibody
Dock4 antibody
DOCK4_HUMAN antibody
KIAA0716 antibody
Images
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ICC staining DOCK4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining DOCK4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining DOCK4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"