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Dicer Recombinant Rabbit Monoclonal Antibody [PSH19-54]

Usd: 385

Specification

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Catalog# HA724080

Dicer Recombinant Rabbit Monoclonal Antibody [PSH19-54]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Dicer Recombinant Rabbit Monoclonal Antibody [PSH19-54]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 219 kDa

Positive Control

HeLa cell lysate, Daudi cell lysate, MCF7 cell lysate, 293T cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

PSH19-54

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. Being part of the RNase III family, Dicer cleaves double-stranded RNA (dsRNA) and pre-microRNA (pre-miRNA) into short double-stranded RNA fragments called small interfering RNA and microRNA, respectively. These fragments are approximately 20–25 base pairs long with a two-base overhang on the 3′-end. Dicer facilitates the activation of the RNA-induced silencing complex (RISC), which is essential for RNA interference. RISC has a catalytic component Argonaute, which is an endonuclease capable of degrading messenger RNA (mRNA).

Background References

1. Poirier EZ et al. An isoform of Dicer protects mammalian stem cells against multiple RNA viruses. Science. 2021 Jul

2. Wang Q et al. Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation. Science. 2021 Nov

Subcellular Location

Cytoplasm, perinuclear region.

UNIPROT

SwissProt: Q9UPY3 Human

SwissProt: Q8R418 Mouse

Entrez Gene: 299284 Rat

Synonyms

DCR antibody

DCR1 antibody

Dicer 1 ribonuclease III antibody

Dicer 1 ribonuclease type III antibody

Dicer antibody

DICER_HUMAN antibody

DICER1 antibody

Double-strand-specific ribonuclease antibody

Endoribonuclease Dicer antibody

Helicase MOI antibody

Expand

Images

  • Western blot analysis of Dicer on different lysates with Rabbit anti-Dicer antibody (<a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Daudi cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: PC-12 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 219 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Dicer on different lysates with Rabbit anti-Dicer antibody (HA724080) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Daudi cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: PC-12 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 219 kDa
    Observed band size: 250 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724080) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Dicer on NIH/3T3 cell lysates with Rabbit anti-Dicer antibody (<a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 219 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 1 minute 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Dicer on NIH/3T3 cell lysates with Rabbit anti-Dicer antibody (HA724080) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 219 kDa
    Observed band size: 250 kDa

    Exposure time: 1 minute 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724080) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Dicer was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA724080" style="font-weight: bold;text-decoration: underline;">HA724080</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 2 minutes 6 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Dicer was immunoprecipitated from 0.2 mg HeLa cell lysate with HA724080 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724080 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA724080 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA724080 in HeLa cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 2 minutes 6 seconds; ECL: K1801

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