Drebrin Rabbit Polyclonal Antibody
Catalog# ER1902-55
Drebrin Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
FC
-
Human
-
unconjugated
Overview
Product Name
Drebrin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human Drebrin aa 275-468 / 649.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 71 kDa
Positive Control
A549 cell lysate, PC-3M cell lysate, HepG2 cell lysate, 293 cell lysate, Siha cell lysate, human lung cancer tissue, human kidney tissue, SHSY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
Target
Function
The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene.Actin cytoskeleton-organizing protein that plays a role in the formation of cell projections . Required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS . Plays a role in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to the dendritic spines (By similarity). Involved in memory-related synaptic plasticity in the hippocampus (By similarity).
Background References
1. Perez-Martinez M.et.al.F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse.J. Cell Sci. 123:1160-1170(2010).
Tissue Specificity
Expressed in the brain, with expression in the molecular layer of the dentate gyrus, stratum pyramidale, and stratum radiatum of the hippocampus (at protein level). Also expressed in the terminal varicosities distributed along dendritic trees of pyramidal cells in CA4 and CA3 of the hippocampus (at protein level). Expressed in pyramidal cells in CA2, CA1 and the subiculum of the hippocampus (at protein level). Expressed in peripheral blood lymphocytes, including T-cells (at protein level). Expressed in the brain. Expressed in the heart, placenta, lung, skeletal muscle, kidney, pancreas, skin fibroblasts, gingival fibroblasts and bone-derived cells (Ref.2).
Subcellular Location
Cell junction, Cell projection, Cytoplasm.
UNIPROT
Synonyms
D0S117E antibody
DBN 1 antibody
DBN1 antibody
Developmentally regulated brain protein antibody
Developmentally-regulated brain protein antibody
DKFZp434D064 antibody
DREB_HUMAN antibody
Drebrin 1 antibody
Drebrin antibody
Drebrin E antibody
ExpandImages
-
Western blot analysis of Drebrin on different lysates with Rabbit anti-Drebrin antibody (ER1902-55) at 1/1,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1902-55, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 71 kDa
Observed band size: 110 kDa -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Drebrin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-55, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Drebrin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-55, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Flow cytometric analysis of Drebrin was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-55, 1/100) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Drebrin Recombinant Rabbit Monoclonal Antibody [JE30-20]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
Products with the same target and pathway