E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45] - BSA and Azide free
Catalog# HA750625
E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human E2F4 aa 21-80 / 413.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 44 kDa
Positive Control
293T cell lysate, Raji cell lysate, HeLa cell lysate, Jurkat cell lysate.
Conjugation
unconjugated
Clone Number
PSH0-45
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Background References
1. Xiao W et al. Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy. 2022 Nov
2. Wang Y et al. Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis. Protein Cell. 2022 Oct
Subcellular Location
Nucleus.
UNIPROT
Synonyms
E2F 4 antibody
E2F transcription factor 4 antibody
E2F transcription factor 4 p107/p130 binding antibody
E2F-4 antibody
E2F4 antibody
E2F4_HUMAN antibody
p107/p130 binding protein antibody
Transcription factor E2F 4 antibody
Transcription factor E2F4 antibody
Images
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This data was developed using HA721362, the same antibody clone in a different buffer formulation.
Western blot analysis of E2F4 on different lysates with Rabbit anti-E2F4 antibody (HA721362) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: Raji cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 10 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721362) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
E2F4 Recombinant Rabbit Monoclonal Antibody [JE40-78]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
