EDG3 / S1P3 Recombinant Rabbit Monoclonal Antibody [JE38-31]
Catalog# HA722337
EDG3 / S1P3 Recombinant Rabbit Monoclonal Antibody [JE38-31]
-
WB
-
IP
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
EDG3 / S1P3 Recombinant Rabbit Monoclonal Antibody [JE38-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide corresponding to Human EDG3/S1P3 aa 30-150.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 42 kDa
Positive Control
HepG2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE38-31
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IP
-
1-2μg/sample
Target
Function
Sphingosine-1-phosphate receptor 3 also known as S1PR3 is a human gene which encodes a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P). Hence this receptor is also known as S1P3. This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function.
Background References
1. Fan X et al. Recent advances of the function of sphingosine 1-phosphate (S1P) receptor S1P3. J Cell Physiol. 2021 Mar
2. Magrassi L et al. EDG3 and SHC3 on chromosome 9q22 are co-amplified in human ependymomas. Cancer Lett. 2010 Apr
Subcellular Location
Cell membrane.
Synonyms
EDG 3 antibody
EDG3 antibody
Endothelial differentiation G protein coupled receptor 3 antibody
Endothelial differentiation G-protein coupled receptor 3 antibody
Endothelial differentiation gene 3 antibody
Endothelial differentiation sphingolipid G protein coupled receptor 3 antibody
FLJ37523 antibody
FLJ93220 antibody
G protein coupled receptor endothelial differentiation gene 3 antibody
LPB 3 antibody
ExpandImages
-
Western blot analysis of EDG3 / S1P3 on different lysates with Rabbit anti-EDG3 / S1P3 antibody (HA722337) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722337) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
EDG3 / S1P3 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA722337 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722337 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (input)
Lane 2: HA722337 IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of HA722337 in HepG2 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 minutes 20 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
