Human/Mouse/Rat Irisin enzyme-linked immunoassay kit
Catalog# EH0081
Human/Mouse/Rat Irisin enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
-
Mouse
-
Rat
Standard curve range
-
15.63~1000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Human/Mouse/Rat Irisin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.60 pg/ml (50 μl)；9.39 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0282 | 0.0291 | 0.0287 | |
| 15.63 | 0.0402 | 0.0405 | 0.0404 | 0.0117 |
| 31.25 | 0.0657 | 0.0541 | 0.0599 | 0.0313 |
| 62.50 | 0.0989 | 0.0921 | 0.0955 | 0.0669 |
| 125.00 | 0.1959 | 0.1961 | 0.1960 | 0.1674 |
| 250.00 | 0.4564 | 0.4599 | 0.4582 | 0.4295 |
| 500.00 | 1.1830 | 1.2010 | 1.1920 | 1.1634 |
| 1000.00 | 2.9400 | 2.9900 | 2.9650 | 2.9364 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 21.5 | 97.1 | 301.0 | 19.5 | 94.8 | 305.4 |
| Standard Deviation | 0.6 | 0.9 | 6.7 | 1.2 | 1.6 | 6.3 |
| Coefficient of Variation (%) | 2.7 | 1.0 | 2.2 | 5.9 | 1.7 | 2.1 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.16-41.72 | 100 | 21.44 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human,Mouse,Rat
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Fibronectin type III domain containing 5
Fibronectin type III domain-containing protein 5
Fibronectin type III repeat containing protein 2
Fibronectin type III repeat-containing protein 2
FNDC 5
Fndc5
FNDC5_HUMAN
FRCP2
irisin