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Human/Mouse/Rat Irisin enzyme-linked immunoassay kit (EH0081)

Price: Regular price $499.00

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Catalog# EH0081

Assay type Sandwich ELISA

Standard curve range 15.63~1000 pg/ml

Sensitivity 2.60 pg/ml (50 μl)；9.39 pg/ml (10 μl)

Reacts with

  • Human

  • Mouse

  • Rat

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02820.02910.0287
15.630.04020.04050.04040.0117
31.250.06570.05410.05990.0313
62.500.09890.09210.09550.0669
125.000.19590.19610.19600.1674
250.000.45640.45990.45820.4295
500.001.18301.20101.19201.1634
1000.002.94002.99002.96502.9364

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)21.597.1301.019.594.8305.4
Standard Deviation0.60.96.71.21.66.3
Coefficient of Variation(%)2.71.02.25.91.72.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum303.16-41.7210021.44

Sensitivity

2.60 pg/ml (50 μl)；9.39 pg/ml (10 μl)

Standard curve range

15.63~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Irisin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 86% to 118% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human/Mouse/Rat Irisin enzyme-linked immunoassay kit (EH0081)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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