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GFP Mouse Monoclonal Antibody [2-D8]

Usd: 350

Specification

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Catalog# EM30501

GFP Mouse Monoclonal Antibody [2-D8]

Application

  • WB

  • IF-Cell

  • ELISA

  • FC

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GFP Mouse Monoclonal Antibody [2-D8]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant full length protein of Aequorea victoria GFP.

Species Reactivity

Species independent

Validated Applications

WB, IF-Cell, ELISA, FC, IP

Molecular Weight

Predicted band size: 27 kDa

Positive Control

Transfected K562 with GFP.

Conjugation

unconjugated

Clone Number

2-D8

RRID

AB_3068992

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:200

  • IP

  • 2-5 µg/ml.

Target

Function

Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.

Background References

1. "Primary structure of the Aequorea victoria green-fluorescent protein."Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J.Gene 111:229-233(1992)

2. "A molecular thermometer based on fluorescent protein blinking."Wong F.H., Banks D.S., Abu-Arish A., Fradin C.J. Am. Chem. Soc. 129:10302-10303(2007)

Sequence Similarity

Belongs to the GFP family.

Tissue Specificity

Photocytes.

Post-translational Modification

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

UNIPROT

SwissProt: P42212 Aequorea victoria

Synonyms

GFP antibody

Green fluorescent protein antibody

yfp antibody

Images

  • Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.

    Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.

  • ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with <a href="/products/EM30501" style="font-weight: bold;text-decoration: underline;">EM30501</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/ET1604-25" style="font-weight: bold;text-decoration: underline;">ET1604-25</a> at 1/1000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: GFP Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/M1210-1" style="font-weight: bold;text-decoration: underline;">M1210-1</a> IP in GFP Tag fusion protein lysate.<br />Lane 3: <a href="/products/EM30501" style="font-weight: bold;text-decoration: underline;">EM30501</a> IP in GFP Tag fusion protein lysate.<br />Lane 4: Mouse IgG instead of <a href="/products/EM30501" style="font-weight: bold;text-decoration: underline;">EM30501</a> in GFP Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with EM30501 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1604-25 at 1/1000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

    Lane 1: GFP Tag fusion protein lysate (input).
    Lane 2: M1210-1 IP in GFP Tag fusion protein lysate.
    Lane 3: EM30501 IP in GFP Tag fusion protein lysate.
    Lane 4: Mouse IgG instead of EM30501 in GFP Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation