Skip to content

E tag Mouse Monoclonal Antibody [B2-E2]

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# EM50104

E tag Mouse Monoclonal Antibody [B2-E2]

Application

  • WB

  • ELISA

  • IP

  • IF-Cell

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

E tag Mouse Monoclonal Antibody [B2-E2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide GAPVPYPDPLEPR-KLH.

Species Reactivity

Species independent

Validated Applications

WB, ELISA, IP, IF-Cell

Positive Control

E tagged recombinant protein.

Conjugation

unconjugated

Clone Number

B2-E2

RRID

AB_3069002

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:20,000

  • ELISA

  • Use at an assay dependent concentration.

  • IF-Cell

  • Use at an assay dependent concentration.

  • IP

  • Use at an assay dependent concentration.

Target

Function

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. E-tag is an epitope tag composed of a 13 residue peptide. This antibody recognizes the E-tag fused to either the amino- or carboxy-terminus of targeted proteins.

Background References

1. Chavanayarn C. et al. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel). 4:554-67 (2012)

2. Leotoing L, et al. A20-binding inhibitor of nuclear factor-kappaB (NF-kappaB)-2 (ABIN-2) is an activator of inhibitor of NF-kappaB (IkappaB) kinase alpha (IKKalpha)-mediated NF-kappaB transcriptional activity. J Biol Chem. 286:32277-88 (2011).

Synonyms

E epitope tag antibody

GAPVPYPDPLEPR epitope tag antibody

GAPVPYPDPLEPR tag antibody

Images

  • Western blot analysis of E tag on E-tagged recombinant protein using anti-E tag antibody at 1/20,000 dilution.

    Western blot analysis of E tag on E-tagged recombinant protein using anti-E tag antibody at 1/20,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation