E tag Mouse Monoclonal Antibody [B2-E2] (EM50104)
Catalog# EM50104
Mouse Monoclonal to E tag
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WB
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ELISA
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IP
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IF-Cell
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Species independent
Specifications
Product Type
Mouse monoclonal primary
Product Name
E tag Mouse Monoclonal Antibody [B2-E2] (EM50104)
Immunogen
Synthetic peptide gapvpypdplepr-klh.
Host
Mouse
Positive Control
E tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
B2-E2
RRID
APPLICATION DILUTION
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WB
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1:20,000
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ELISA
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Use at an assay dependent concentration.
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IF-Cell
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Use at an assay dependent concentration.
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Isotype
IgG1
TARGET
PROTEIN NAME
E tag
SYNONYMS
E epitope tag antibody;GAPVPYPDPLEPR epitope tag antibody;GAPVPYPDPLEPR tag antibody
FUNCTION
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. E-tag is an epitope tag composed of a 13 residue peptide. This antibody recognizes the E-tag fused to either the amino- or carboxy-terminus of targeted proteins.
CITATIONS
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Guiying Li
Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1α
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WB
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CO-IP
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HEK293
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HepG2
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RAW264.7
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and HUVEC cell
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Citation
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