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EMA Mouse Monoclonal Antibody

Usd: 278

Specification

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Catalog# IRS253MS

EMA Mouse Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

EMA Mouse Monoclonal Antibody

Antibody Type

Mouse Monoclonal Antibody

Species Reactivity

Human

Validated Applications

mIHC

Molecular Weight

Predicted band size: 122 kDa

Positive Control

Human pancreas tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100

Target

Function

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

Background References

1. Taylor-Papadimitriou J. et. al. Latest developments in MUC1 immunotherapy. Biochem Soc Trans. 2018 Jun

2. Kato K. et. al. MUC1: The First Respiratory Mucin with an Anti-Inflammatory Function. J Clin Med. 2017 Nov

Subcellular Location

Apical cell membrane. Secreted. Nucleus, Cell membrane, Cytoplasm.

UNIPROT

SwissProt:P15941 Human

Synonyms

ADMCKD antibody

ADMCKD1 antibody

Breast carcinoma associated antigen DF3 antibody

Breast carcinoma-associated antigen DF3 antibody

CA 15-3 antibody

CA15 3 antibody

CA15 3 antigen antibody

CA15-3 antibody

CA15.3 antibody

Cancer antigen 15-3 antibody

Expand

Images

  • mIHC analysis of human pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-EMA antibody (<a href="/products/IRS253MS" style="font-weight: bold;text-decoration: underline;">IRS253MS</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900809" style="font-weight: bold;text-decoration: underline;">900809</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-EMA antibody (IRS253MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"