miRFP703 Rabbit Polyclonal Antibody
Catalog# ER1706-42
miRFP703 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Rhodopseudomonas palustris
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unconjugated
Overview
Product Name
miRFP703 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within rhodopseudomonas palustris miRFP703 aa 1-355 / 731.
Species Reactivity
Rhodopseudomonas palustris
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 81 kDa
Positive Control
miRFP recombinant protein.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
Target
Function
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Background References
1. Duclos CM et al. Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling. Cell Death Discov 3:16100 (2017).
2. Shcherbakova DM et al. Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Nat Commun 7:12405 (2016).
Images
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Western blot analysis of miRFP on miRFP recombinant protein lysate using anti-miRFP antibody at 1/5,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"