ASK1 Rabbit Polyclonal Antibody
Catalog# ER1802-84
ASK1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
ASK1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide of C-terminal human ASK1.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 155 kDa
Positive Control
Human skeletal muscle tissue lysate, human colon cancer tissue, mouse testis tissue, PANC-1.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.
Background References
暂无
Sequence Similarity
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Tissue Specificity
Abundantly expressed in heart and pancreas.
Post-translational Modification
Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5.; Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination leading to proteasomal degradation.
Subcellular Location
Cytoplasm, Endoplasmic reticulum.
Synonyms
Apoptosis signal regulating kinase 1 antibody
Apoptosis signal-regulating kinase 1 antibody
ASK 1 antibody
ASK-1 antibody
ASK1 antibody
M3K5 antibody
M3K5_HUMAN antibody
MAP/ERK kinase kinase 5 antibody
MAP3K5 antibody
MAPK/ERK kinase kinase 5 antibody
ExpandImages
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Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution.
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ICC staining ASK1 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ASK1 Recombinant Rabbit Monoclonal Antibody [SU36-06]
Application: WB,IF-Tissue,IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
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