ERO1L Rabbit Polyclonal Antibody
Catalog# ER1908-32
ERO1L Rabbit Polyclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
ERO1L Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human ERO1L: 321-420/468
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
52 kDa
Positive Control
Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-2000
Target
Function
Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
Background References
暂无
Subcellular Location
Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
Synonyms
Endoplasmic oxidoreductin 1 like protein antibody
Endoplasmic oxidoreductin-1-like protein antibody
Endoplasmic reticulum oxidoreductase 1 alpha antibody
Endoplasmic reticulum oxidoreductin 1-like antibody
ERO1 alpha antibody
ERO1 L antibody
ERO1 Lalpha antibody
ERO1 like protein alpha antibody
ERO1-alpha antibody
ERO1-L antibody
ExpandImages
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Sample:
SW480(Human) Cell Lysate at 40 ug
Primary: Anti-ERO1L (ER1908-32) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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