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Phosphotyrosine Recombinant Rabbit Monoclonal Antibody [JA10-49]

Usd: 385

Specification

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Catalog# ET1704-20

Phosphotyrosine Recombinant Rabbit Monoclonal Antibody [JA10-49]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IP

  • FC

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phosphotyrosine Recombinant Rabbit Monoclonal Antibody [JA10-49]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

CXXXX-pY-XXXXNH2.

Species Reactivity

Species independent

Validated Applications

WB, IF-Cell, IF-Tissue, IP, FC

Positive Control

A431 cells lysates treated with Sodium metavanadate.

Conjugation

unconjugated

Clone Number

JA10-49

RRID

AB_3070471

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:50-1:200

  • FC

  • 1:10-1:50

  • IF-Cell

  • 1:50

  • IF-Tissue

  • 1:50

Target

Function

Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals. Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Background References

1. Nakamura S et al. Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation. Sci Rep 5:13474 (2015).

2. Zalk R et al. Structure of a mammalian ryanodine receptor. Nature 517:44-9 (2015).

UNIPROT

SwissProt:Q7Z2X4 Human

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phosphotyrosine on A431 cells lysates treated (Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.

    ☑ Cell treatment (CT)

    Western blot analysis of Phosphotyrosine on A431 cells lysates treated (Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ☑ Cell treatment (CT)

    ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation