Eph receptor A2 Recombinant Rabbit Monoclonal Antibody [PSH09-21]
Overview
Product Name
Eph receptor A2 Recombinant Rabbit Monoclonal Antibody [PSH09-21]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human EPHA2 aa 10-550 / 976
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IP, FC
Target Molecular Weight
Predicted band size: 108 kDa
Positive Control
A549 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A549, HeLa.
Conjugation
unconjugated
Clone Number
PSH09-21
Reactivity Data
| WB | IF-Cell | IP | FC | |
|---|---|---|---|---|
| Human |
|
|
||
| Mouse |
|
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:100
-
IP
-
1-2μg/sample
-
FC
-
1:1,000
Target
Function
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.
Background References
1. Xiaojun S ,Ryan L ,J C H , et al.Time-resolved live-cell spectroscopy reveals EphA2 multimeric assembly.[J].Science (New York, N.Y.),2023,eadg5314-eadg5314.
2. Qiu P ,Ling C ,Wei W , et al.EPH receptor A2 governs a feedback loop that activates Wnt/β-catenin signaling in gastric cancer.[J].Cell death & disease,2018,9(12):1146.
Subcellular Location
Cell membrane, Cell projection, ruffle membrane, lamellipodium membrane, Cell junction, focal adhesion.
Synonyms
ARCC2 antibody
AW545284 antibody
CTPA antibody
CTPP1 antibody
CTRCT6 antibody
EC 2.7.10.1 antibody
Eck antibody
Eph receptor A2 antibody
EPHA2 antibody
EPHA2_HUMAN antibody
ExpandImages
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Western blot analysis of Eph receptor A2 on different lysates with Rabbit anti-Eph receptor A2 antibody (HA723079) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 108 kDa
Observed band size: 130 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723079) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A549 cells labeling Eph receptor A2 with Rabbit anti-Eph receptor A2 antibody (HA723079) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor A2 antibody (HA723079) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Eph receptor A2 was immunoprecipitated from 0.2 mg A549 cell lysate with HA723079 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723079 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (input)
Lane 2: HA723079 IP in A549 cell lysate
Lane 3: Rabbit IgG instead of HA723079 in A549 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 59 seconds; ECL: K1801 -
Flow cytometric analysis of A549 cells labeling Eph receptor A2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723079, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Citation
-
Artesunate Suppresses Choroidal Melanoma Vasculogenic Mimicry Formation and Angiogenesis via the Wnt/CaMKII Signaling Axis
Journal: Frontiers In Oncology
DOI:
IF: 6.24
Application: WB
Reactivity: Human
Publish date: 2021 Aug
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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