Erk1 (pT202+pY204) + Erk2 (pT185+pY187) Recombinant Rabbit Monoclonal Antibody [PD00-38]
Catalog# HA724560
Erk1 (pT202+pY204) + Erk2 (pT185+pY187) Recombinant Rabbit Monoclonal Antibody [PD00-38]
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WB
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IHC-P
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IF-Cell
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Human
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unconjugated
Overview
Product Name
Erk1 (pT202+pY204) + Erk2 (pT185+pY187) Recombinant Rabbit Monoclonal Antibody [PD00-38]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human aa 166-215 / 379.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 41/43 kDa
Positive Control
A431 cell lysate, SK-Br-3 cell lysate, human human thyroid carcinoma tissue, MCF-7.
Conjugation
unconjugated
Clone Number
PD00-38
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:5,000
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IF-Cell
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1:50
Target
Function
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Background References
1. Wang ZH et al. Sunitinib mesylate inhibits proliferation of human colonic stromal fibroblasts in vitro and in vivo. J Zhejiang Univ Sci B 15:701-12 (2014).
2. Assi J et al. Transglutaminase 2 overexpression in tumor stroma identifies invasive ductal carcinomas of breast at high risk of recurrence. PLoS One 8:e74437 (2013).
Sequence Similarity
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Post-translational Modification
Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
ERK 1 antibody
ERK 2 antibody
ERK-1 antibody
ERK-2 antibody
ERK1 antibody
erk1/2 antibody
ERK2 antibody
ERT1 antibody
ERT2 antibody
Extracellular signal regulated kinase 1 antibody
ExpandImages
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Western blot analysis of Erk1 (pT202+pY204) + Erk2 (pT185+pY187) on different lysates with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: SK-Br-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 41/43 kDa
Observed band size: 41/43 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724560) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724560) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of MCF-7 cells labeling Erk1 (pT202+pY204) + Erk2 (pT185+pY187) with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Erk1 (pT202+pY204) + Erk2 (pT185+pY187) antibody (HA724560) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Phospho-Erk1 (T202)+Erk2 (T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,FC,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
