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Estrogen Receptor alpha Mouse Monoclonal Antibody [E8-A6]

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Catalog# EM1708-51

Estrogen Receptor alpha Mouse Monoclonal Antibody [E8-A6]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Rabbit

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Estrogen Receptor alpha Mouse Monoclonal Antibody [E8-A6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Purified recombinant fragment of human ESR1 (AA: 2-185) expressed in E. Coli.

Species Reactivity

Human, Mouse, Rat, Rabbit

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

66 kDa

Conjugation

unconjugated

Clone Number

E8-A6

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles.

Storage Buffer

Purified antibody in PBS with 0.05% sodium azide

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:500

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:200-1:400

Target

Function

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

Background References

1. Jeon YW et al. Synergistic effect between celecoxib and luteolin is dependent on estrogen receptor in human breast cancer cells. Tumour Biol 36(8):6349-59 (2015).

2. Alluri PG et al. Estrogen receptor mutations and their role in breast cancer progression. Breast Cancer Res 16(6):494 (2014).

Subcellular Location

Nucleus, cytoplasm.

UNIPROT

SwissProt: P03372 Human

SwissProt: P06211 Rat

Synonyms

7*/654 isoform antibody

7*/819 2 isoform antibody

7*/822 isoform antibody

8*/901 isoform antibody

8*/941 isoform antibody

DKFZp686N23123 antibody

ER alpha antibody

ER antibody

ER-alpha antibody

Era antibody

Expand

Images

  • Western blot analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.

    Western blot analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.

  • Western blot analysis using ESR1 mouse mAb against PC-12(1)Raw264.7(2)NIH/3T3(3)NRK(4)C2C12(5)C6(6)L1210(7)F9(8)COS-7(9) cell lysate.

    Western blot analysis using ESR1 mouse mAb against PC-12(1)Raw264.7(2)NIH/3T3(3)NRK(4)C2C12(5)C6(6)L1210(7)F9(8)COS-7(9) cell lysate.

  • Immunofluorescence analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    Immunofluorescence analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Immunofluorescence analysis of COS7 cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    Immunofluorescence analysis of COS7 cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ESR1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ESR1 mouse mAb with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ESR1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ESR1 mouse mAb with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Mouse testicles(A)Mouse kidney(B)Mouse cerebellum(C) using ESR1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded Mouse testicles(A)Mouse kidney(B)Mouse cerebellum(C) using ESR1 mouse mAb with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Rat testicles(A)Rat kidney(B)Rat cerebellum(C) using ESR1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded Rat testicles(A)Rat kidney(B)Rat cerebellum(C) using ESR1 mouse mAb with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A)Rabbit cerebellum(B)Rabbit kidney(C) using ESR1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A)Rabbit cerebellum(B)Rabbit kidney(C) using ESR1 mouse mAb with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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