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F4/80 Rat Monoclonal Antibody [4G4]

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Catalog# RT1212

F4/80 Rat Monoclonal Antibody [4G4]

Application

  • WB

  • IF

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

F4/80 Rat Monoclonal Antibody [4G4]

Antibody Type

Rat Monoclonal Antibody

Immunogen

peptide

Species Reactivity

Human, Mouse

Validated Applications

WB, IF, IHC-P, FC

Molecular Weight

160 kDa

Conjugation

unconjugated

Clone Number

4G4

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃

Storage Buffer

1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Isotype

IgG2a

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:100-1:1,000

  • IF

  • 1:50-1:500

  • IHC-P

  • 1:50-1:500

  • FC

  • 1 µg per 1 x 10^6 cells

Target

Function

The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL. These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system. The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin. F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation. The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.

Background References

1. Mouline, CC. et al. 2012. Monocytes differentiation upon treatment with a peptide corresponding to the C-terminus of activated T cell-expressed Tirc7 protein. J. Cell. Physiol. 227: 3088-3098.

2. Zheng, JL. et al. 2012. Cannabinoid receptor type 2 is time-dependently expressed during skin wound healing in mice. Int. J. Legal Med. 126: 807-814.

Subcellular Location

Cell membrane

UNIPROT

SwissProt: Q61549 Mouse

Synonyms

Adgre1 antibody

Adhesion G protein-coupled receptor E1 antibody

AGRE1_HUMAN antibody

EGF-like module receptor 1 antibody

EGF-like module-containing mucin-like hormone receptor-like 1 antibody

EMR1 hormone receptor antibody

Images

  • F4/80 Antibody: RT1212. Immunoperoxidase staining of formalin fixed, paraffin-embedded liver tissue of normal mouse fed with low fat diet (A) or choline-deficient high fat diet (B) showing F4/80 staining in Kupffer cells.

    F4/80 Antibody: RT1212. Immunoperoxidase staining of formalin fixed, paraffin-embedded liver tissue of normal mouse fed with low fat diet (A) or choline-deficient high fat diet (B) showing F4/80 staining in Kupffer cells.

  • F4/80 Antibody: RT1212. Immunoperoxidase staining of formalin fixed, paraffin-embedded liver tissue of normal mouse fed with low fat diet (A) or choline-deficient high fat diet (B) showing F4/80 staining in Kupffer cells.

    F4/80 Antibody: RT1212. Immunoperoxidase staining of formalin fixed, paraffin-embedded liver tissue of normal mouse fed with low fat diet (A) or choline-deficient high fat diet (B) showing F4/80 staining in Kupffer cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation