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FADD Recombinant Rabbit Monoclonal Antibody [JE32-76]

Usd: 385

Specification

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Catalog# HA722140

FADD Recombinant Rabbit Monoclonal Antibody [JE32-76]

Application

  • WB

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FADD Recombinant Rabbit Monoclonal Antibody [JE32-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Mouse FADD aa 106-205 / 205.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 23 kDa

Positive Control

NIH/3T3 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse lung tissue lysate, mouse colon tissue lysate.

Conjugation

unconjugated

Clone Number

JE32-76

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

FAS-associated death domain protein, also called MORT1, is encoded by the FADD gene on the 11q13.3 region of chromosome 11 in humans. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development.

Background References

1. Schwarzer R et al. FADD and Caspase-8 Regulate Gut Homeostasis and Inflammation by Controlling MLKL- and GSDMD-Mediated Death of Intestinal Epithelial Cells. Immunity. 2020 Jun

2. Rodriguez DA et al. Caspase-8 and FADD prevent spontaneous ZBP1 expression and necroptosis. Proc Natl Acad Sci U S A. 2022 Oct

Subcellular Location

Cytoplasm, cytosol, neuron projection, nucleus, plasma membrane, ripoptosome.

UNIPROT

SwissProt: Q61160 Mouse

Entrez Gene: 266610 Rat

Synonyms

FADD antibody

FADD protein antibody

FADD_HUMAN antibody

Fas (TNFRSF6) associated via death domain antibody

Fas associated via death domain antibody

Fas associating death domain containing protein antibody

Fas associating protein antibody

Fas associating protein with death domain antibody

Fas TNFRSF6 associated via death domain antibody

FAS-associated death domain protein antibody

Expand

Images

  • Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (<a href="/products/HA722140" style="font-weight: bold;text-decoration: underline;">HA722140</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate (10 µg/Lane)<br />Lane 2: C6 cell lysate (10 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 23 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722140" style="font-weight: bold;text-decoration: underline;">HA722140</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA722140) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate (10 µg/Lane)
    Lane 2: C6 cell lysate (10 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (20 µg/Lane)

    Predicted band size: 23 kDa
    Observed band size: 27 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722140) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (<a href="/products/HA722140" style="font-weight: bold;text-decoration: underline;">HA722140</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Mouse colon tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 23 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722140" style="font-weight: bold;text-decoration: underline;">HA722140</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA722140) at 1/1,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Mouse colon tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 23 kDa
    Observed band size: 27 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722140) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation