FOXA1 Mouse Monoclonal Antibody [A2E8]
Catalog# EM1902-34
FOXA1 Mouse Monoclonal Antibody [A2E8]
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WB
-
IHC-P
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FC
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IF-Cell
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Human
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unconjugated
Overview
Product Name
FOXA1 Mouse Monoclonal Antibody [A2E8]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within N-terminal human FOXA1.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC, IF-Cell
Molecular Weight
Predicted band size: 49 kDa
Positive Control
LNCaP cell lysates, LNCaP, human prostate tissue, MCF-7.
Conjugation
unconjugated
Clone Number
A2E8
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:500
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FC
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1:50-1:100
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IF-Cell
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1:100
Target
Function
Forkhead box protein A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha (HNF-3A), is a protein that in humans is encoded by the FOXA1 gene. FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA1 in breast cancer is highly correlated with ERα+, GATA3+, and PR+ protein expression as well as endocrine signaling. FOXA1 acts as a pioneer factor for ERa in ERα+ breast cancer, and its expression might identify ERα+ cancers that undergo rapid reprogramming of ERa signaling that is associated with poor outcomes and treatment resistance. Conversely, in ERα− breast cancer FOXA1 is highly correlated with low-grade morphology and improved disease free survival. FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERα− cancers may identify a subset of tumors that is responsive to other endocrine therapies such as androgen receptor antagonist treatment.
Background References
1. Adams EJ. et. al. FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Nature. 2019 Jul
2. Parolia A. et. al. Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer. Nature. 2019 Jul
Subcellular Location
Nucleus.
UNIPROT
Synonyms
forkhead box A1 antibody
Forkhead box protein A1 antibody
FOX A1 antibody
FOXA1 antibody
FOXA1_HUMAN antibody
hepatocyte nuclear factor 3 alpha antibody
Hepatocyte nuclear factor 3-alpha antibody
HNF 3A antibody
HNF-3-alpha antibody
HNF-3A antibody
ExpandImages
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Western blot analysis of FOXA1 on LNCaP cell lysates with Mouse anti-FOXA1 antibody (EM1902-34) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of LNCaP cells labeling FOXA1 with Mouse anti-FOXA1 antibody (EM1902-34) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-FOXA1 antibody (EM1902-34) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-FOXA1 antibody (EM1902-34) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-34) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of FOXA1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"