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FPR2 Recombinant Rabbit Monoclonal Antibody [PSH21-63]

Usd: 385

Specification

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Catalog# HA724230

FPR2 Recombinant Rabbit Monoclonal Antibody [PSH21-63]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

FPR2 Recombinant Rabbit Monoclonal Antibody [PSH21-63]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human FPR2 aa 1-50.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 39 kDa

Positive Control

HL-60 cell lysate, THP-1 cell lysate, J774A.1 cell lysate, RAW264.7 cell lysate, Mouse brain tissue lysate, Mouse bone marrow tissue lysate, Rat brain tissue lysate, THP-1.

Conjugation

unconjugated

Clone Number

PSH21-63

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:500

  • FC

  • 1:1,000

Target

Function

N-formyl peptide receptor 2 (FPR2) is a G-protein coupled receptor (GPCR) located on the surface of many cell types of various animal species. The human receptor protein is encoded by the FPR2 gene and is activated to regulate cell function by binding any one of a wide variety of ligands including not only certain N-Formylmethionine-containing oligopeptides such as N-Formylmethionine-leucyl-phenylalanine (FMLP) but also the polyunsaturated fatty acid metabolite of arachidonic acid, lipoxin A4 (LXA4) and long chain Ceramide. Because of its interaction with lipoxin A4, FPR2 is also commonly named the ALX/FPR2 or just ALX receptor.

Background References

1. Wu MY et al. Enhancement of efferocytosis through biased FPR2 signaling attenuates intestinal inflammation. EMBO Mol Med. 2023 Dec

2. Xu X et al. Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway. J Neuroinflammation. 2021 May

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P25090 Human

SwissProt: O88536 Mouse

Entrez Gene: 690158 Rat

Synonyms

ALXR antibody

FMLP-R-I antibody

FMLP-R-II antibody

FMLP-related receptor I antibody

FMLPX antibody

Formyl peptide receptor 2 antibody

Formyl peptide receptor related antibody

Formyl peptide receptor-like 1 antibody

FPR2 antibody

FPR2_HUMAN antibody

Expand

Images

  • Western blot analysis of FPR2 on different lysates with Rabbit anti-FPR2 antibody (<a href="/products/HA724230" style="font-weight: bold;text-decoration: underline;">HA724230</a>) at 1/5,000 dilution.<br /><br />Lane 1: HL-60 cell lysate (20 µg/Lane)<br />Lane 2: THP-1 cell lysate (20 µg/Lane)<br />Lane 3: J774A.1 cell lysate (20 µg/Lane)<br />Lane 4: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 6: Mouse bone marrow tissue lysate (40 µg/Lane)<br />Lane 7: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 39 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724230" style="font-weight: bold;text-decoration: underline;">HA724230</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FPR2 on different lysates with Rabbit anti-FPR2 antibody (HA724230) at 1/5,000 dilution.

    Lane 1: HL-60 cell lysate (20 µg/Lane)
    Lane 2: THP-1 cell lysate (20 µg/Lane)
    Lane 3: J774A.1 cell lysate (20 µg/Lane)
    Lane 4: RAW264.7 cell lysate (20 µg/Lane)
    Lane 5: Mouse brain tissue lysate (40 µg/Lane)
    Lane 6: Mouse bone marrow tissue lysate (40 µg/Lane)
    Lane 7: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 39 kDa
    Observed band size: 35 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724230) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of THP-1 cells labeling FPR2 with Rabbit anti-FPR2 antibody (<a href="/products/HA724230" style="font-weight: bold;text-decoration: underline;">HA724230</a>) at 1/500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FPR2 antibody (<a href="/products/HA724230" style="font-weight: bold;text-decoration: underline;">HA724230</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of THP-1 cells labeling FPR2 with Rabbit anti-FPR2 antibody (HA724230) at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FPR2 antibody (HA724230) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of THP-1 cells labeling FPR2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA724230" style="font-weight: bold;text-decoration: underline;">HA724230</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of THP-1 cells labeling FPR2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA724230, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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