Factor XIIIa Recombinant Rabbit Monoclonal Antibody [PD00-08]
Catalog# HA721148
Factor XIIIa Recombinant Rabbit Monoclonal Antibody [PD00-08]
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WB
-
IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
Factor XIIIa Recombinant Rabbit Monoclonal Antibody [PD00-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Factor XIIIa C terminal.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 83 kDa
Positive Control
Human placenta tissue lysates, human lymph nodes tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
PD00-08
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:1,000-1:2,000
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IF-Tissue
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1:1,000
Target
Function
This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.Factor XIIIa has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases +), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
Background References
1. Calik M. et. al. Prognostic Value of TRPM7 Expression and Factor XIIIa-Expressing Tumor-Associated Macrophages in Gastric Cancer. Gastroenterol Res Pract. 2021 Dec
2. Al-Horani RA. et. al. Factor XIIIa inhibitors as potential novel drugs for venous thromboembolism. Eur J Med Chem. 2020 Aug
Subcellular Location
Secreted, Cytoplasm.
UNIPROT
Synonyms
bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
Coagulation factor XIII A chain antibody
Coagulation factor XIII A1 polypeptide antibody
Coagulation factor XIII A1 subunit antibody
Coagulation factor XIII, A polypeptide antibody
Coagulation factor XIIIa antibody
F13A antibody
F13A_HUMAN antibody
F13a1 antibody
Factor XIIIA antibody
ExpandImages
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Western blot analysis of Factor XIIIa on human placenta tissue lysates with Rabbit anti-Factor XIIIa antibody (HA721148) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721148) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Factor XIIIa antibody (HA721148) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Factor XIIIa antibody (HA721148) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721148) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: lymph nodes
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Factor XIIIa Rabbit Polyclonal Antibody
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Factor XIIIa Recombinant Rabbit Monoclonal Antibody [PSH21-18] - BSA and Azide free
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
