Fast Myosin Skeletal Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH05-12] - BSA and Azide free
Catalog# HA750985
Fast Myosin Skeletal Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH05-12] - BSA and Azide free
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Fast Myosin Skeletal Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH05-12] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Fast Myosin Skeletal Heavy chain aa
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 223 kDa
Positive Control
Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
Conjugation
unconjugated
Clone Number
PSH05-12
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:2,000-1:10,000
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IF-Tissue
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1:400-1:2,000
Target
Function
Myosin-1, also known as 'striated muscle myosin heavy chain 1', is a protein that in humans is encoded by the MYH1 gene. This gene is most highly expressed in fast type IIX/D muscle fibres of vertebrates and encodes a protein found uniquely in striated muscle; it is a class II myosin with a long coiled coil tail that dimerizes and should not be confused with 'Myosin 1' encoded by the MYO1 family of genes (MYO1A-MYO1H). Class I MYO1 genes function in many cell types throughout biology and are single-headed membrane-binding myosins that lack a long coiled coil tail. Myosin is a major contractile protein that converts chemical energy into mechanical energy through the hydrolysis of ATP. Class II Myosins are hexameric proteins composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals, at least ten different myosin heavy chain (MYH) isoforms have been described from striated, smooth, but rarely in non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development.
Background References
1. Alsaif HS et al. MYH1 is a candidate gene for recurrent rhabdomyolysis in humans. Am J Med Genet A. 2021 Jul
2. de Albuquerque AL et al. Prevalence of the E321G MYH1 variant in Brazilian Quarter Horses. Equine Vet J. 2022 Sep
Subcellular Location
Cytoplasm, myofibril.
Synonyms
adult 1 antibody
MYH1 antibody
MYH1_HUMAN antibody
MyHC-2x antibody
MyHC-IIx/d antibody
Myosin heavy chain 1 antibody
Myosin heavy chain 2x antibody
Myosin heavy chain antibody
Myosin heavy chain IIx/d antibody
Myosin-1 antibody
ExpandImages
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☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue / human brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA750985) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750985) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue / mouse brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA750985) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750985) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue / rat brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA750985) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750985) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Mouse
Site: skeletal muscle
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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