Fibrinogen Mouse Monoclonal Antibody [D5-B3]
Catalog# M1510-11
Fibrinogen Mouse Monoclonal Antibody [D5-B3]
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IHC-P
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WB
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Human
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unconjugated
Overview
Product Name
Fibrinogen Mouse Monoclonal Antibody [D5-B3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
native protein.
Species Reactivity
Human
Validated Applications
IHC-P, WB
Molecular Weight
Predicted band size: 95/56/52 kDa
Positive Control
HepG2 cell lysates, human liver tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
D5-B3
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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IHC-P
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1:50-1:200
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WB
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1:500-1:2000
Target
Function
The plasma glycoprotein Fibrinogen is synthesized in the liver and comprises three structurally different subunits: a, b and g. Fibrinogen is important in platelet aggregation, the final step of the coagulation cascade (i.e. the formation of Fibrin) and determination of plasma viscosity and erythrocyte aggregation. It is both constitutively expressed and inducible during an acute phase reaction. Hemostasis following tissue injury deploys essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Following vascular injury, Fibrinogen is cleaved by Thrombin to form Fibrin, which is the most abundant component of blood clots. The cleavage products of Fibrinogen regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types.
Background References
1. Spraggon G et al. Crystal structure of a recombinant alphaEC domain from human fibrinogen-420. Proc Natl Acad Sci USA 95:9099-9104 (1998).
2. Kollman JM et al. Crystal structure of human fibrinogen. Biochemistry 48:3877-3886 (2009).
Tissue Specificity
Detected in blood plasma (at protein level).
Post-translational Modification
The alpha chain is normally not N-glycosylated. It is well known that heterologous expression of isolated domains can lead to adventitious protein modifications. Besides, glycosylation at Asn-686 is supported by large-scale glycoproteomics studies (PubMed:16335952 and PubMed:19159218), but the evidence is still quite tenuous. Most likely, Asn-686 is not glycosylated in the healthy human body, or only with low efficiency.; O-glycosylated.; Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.; About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.; Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.; Phosphorylated by FAM20C in the extracellular medium.
Subcellular Location
Secreted.
Synonyms
FGA antibody
Fib2 antibody
FIBA_HUMAN antibody
Fibrinogen A alpha polypeptide antibody
Fibrinogen alpha chain antibody
Fibrinogen B alpha polypeptide antibody
Fibrinogen beta chain antibody
Fibrinogen G alpha polypeptide antibody
Fibrinogen gamma chain antibody
fibrinogen, B beta polypeptide antibody
ExpandImages
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Western blot analysis of Fibrinogen on HepG2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-11, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Fibrinogen alpha chain Recombinant Rabbit Monoclonal Antibody [JE37-07]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
Fibrinogen Mouse Monoclonal Antibody [1-A7]
Application: WB,IHC-P,ELISA
Reactivity: Human
Conjugate: unconjugated
Fibrinogen Rabbit Polyclonal Antibody
Application: WB,IF-Cell,FC,IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
