Frizzled 7 Rabbit Polyclonal Antibody
Catalog# ER1909-03
Frizzled 7 Rabbit Polyclonal Antibody
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Frizzled 7 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Frizzled 7 501-574/574
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
60 KDa
Positive Control
High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-2000
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FC
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3ug/Test
Target
Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Background References
暂无
Subcellular Location
Membrane; Multi-pass membrane protein.
UNIPROT
Synonyms
Frizzled 7
seven transmembrane spanning receptor antibody
frizzled class receptor 7 antibody
Frizzled drosophila homolog of 7 antibody
Frizzled family receptor 7 antibody
Frizzled homolog 7 antibody
Frizzled-7 antibody
Frizzled7 antibody
Fz 7 antibody
Fz-7 antibody
ExpandImages
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Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Frizzled 7 (ER1909-03) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD -
Sample:
HepG2(Human) Cell Lysate at 30 ug
Muscle (Mouse) Lysate at 40 ug
Muscle (Rat) Lysate at 40 ug
Primary: Anti-Frizzled 7 (ER1909-03) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD -
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (ER1909-03)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Frizzled 7 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway