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Fyn Recombinant Rabbit Monoclonal Antibody [JE42-22]

Usd: 385

Specification

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Catalog# HA721577

Fyn Recombinant Rabbit Monoclonal Antibody [JE42-22]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Fyn Recombinant Rabbit Monoclonal Antibody [JE42-22]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Fyn aa 25-74 / 537.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 61 kDa

Positive Control

Rat testis tissue lysate, Mouse testis tissue lysate, Human brain tissue lysate, Mouse lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, 293T cell lysate, NIH/3T3 cell lysate.

Conjugation

unconjugated

Clone Number

JE42-22

RRID

AB_3072690

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Proto-oncogene tyrosine-protein kinase Fyn (p59-FYN, Slk, Syn, MGC45350, Gene ID 2534) is an enzyme that in humans is encoded by the FYN gene. Fyn is a 59-kDa member of the Src family of kinases typically associated with T-cell and neuronal signaling in development and normal cell physiology. Disruptions in these signaling pathways often have implications in the formation of a variety of cancers. By definition as a proto-oncogene, Fyn codes for proteins that help regulate cell growth. Changes in its DNA sequence transform it into an oncogene that leads to the formation of a different protein with implications for normal cell regulation. Fyn is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Background References

1. Du F et al. Fyn Signaling in Ischemia-Reperfusion Injury: Potential and Therapeutic Implications. Mediators Inflamm. 2022 Sep

2. Demuro S et al. GSK-3β, FYN, and DYRK1A: Master Regulators in Neurodegenerative Pathways. Int J Mol Sci. 2021 Aug

Subcellular Location

Cytoplasm, Nucleus, Cell membrane, Perikaryon.

UNIPROT

SwissProt: P06241 Human

SwissProt: P39688 Mouse

SwissProt: Q62844 Rat

Synonyms

C syn protooncogene antibody

Fyn antibody

FYN oncogene related to SRC FGR YES antibody

FYN_HUMAN antibody

OKT3 induced calcium influx regulator antibody

P59 FYN antibody

p59-Fyn antibody

Protein tyrosine kinase fyn antibody

Proto oncogene tyrosine protein kinase fyn antibody

Proto-oncogene c-Fyn antibody

Expand

Images

  • Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution.<br /><br />Lane 1: Rat testis tissue lysate<br />Lane 2: Mouse testis tissue lysate<br />Lane 3: Human brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 61 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution.

    Lane 1: Rat testis tissue lysate
    Lane 2: Mouse testis tissue lysate
    Lane 3: Human brain tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 61 kDa
    Observed band size: 59 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Rat brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 61 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 61 kDa
    Observed band size: 59 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate (20 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 3: Human brain tissue lysate (40 µg/Lane)<br />Lane 4: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 61 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 14 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution.

    Lane 1: 293T cell lysate (20 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 3: Human brain tissue lysate (40 µg/Lane)
    Lane 4: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 61 kDa
    Observed band size: 59 kDa

    Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 14 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Fyn KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 61 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 180 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721577" style="font-weight: bold;text-decoration: underline;">HA721577</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Fyn KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 61 kDa
    Observed band size: 59 kDa

    Exposure time: 180 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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