GABRA2 Recombinant Rabbit Monoclonal Antibody [PSH17-28]
Catalog# HA723915
GABRA2 Recombinant Rabbit Monoclonal Antibody [PSH17-28]
-
IHC-Fr
-
IHC-P
-
WB
-
IP
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
GABRA2 Recombinant Rabbit Monoclonal Antibody [PSH17-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, IHC-P, WB, IP
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
Mouse hippocampus tissue, rat hippocampus tissue, Mouse brain tissue lysate, Rat cerebellum tissue lysate.
Conjugation
unconjugated
Clone Number
PSH17-28
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | IF-Tissue | IP | |
|---|---|---|---|---|---|
| human |
|
|
|
|
|
| mouse |
|
|
|
|
|
| rat |
|
|
|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-Fr
-
1:500
-
IHC-P
-
1:200-1:1,000
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
Target
Function
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. Yu W. et. al. Gabra2 is a genetic modifier of Scn8a encephalopathy in the mouse. Epilepsia. 2020 Dec
2. Goetjen A. et. al. Induced pluripotent stem cell reprogramming-associated methylation at the GABRA2 promoter and chr4p12 GABA(A) subunit gene expression in the context of alcohol use disorder. Am J Med Genet B Neuropsychiatr Genet. 2020 Dec
Subcellular Location
Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Cell projection, dendrite.
Synonyms
GABA A receptor subunit alpha 2 antibody
GABA antibody
GABA(A) receptor subunit alpha 2 antibody
GABA(A) receptor subunit alpha-2 antibody
GABR A2 antibody
GABRA 2 antibody
GABRA2 antibody
GABRA2 protein antibody
Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
Gamma aminobutyric acid A receptor alpha 2 antibody
ExpandImages
-
Application: IHC-Fr
Species: Mouse
Site: hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-GABRA2 antibody (HA723915) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723915) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GABRA2 antibody (HA723915) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723915) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of GABRA2 on different lysates with Rabbit anti-GABRA2 antibody (HA723915) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723915) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
GABRA2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA723915 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723915 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: mouse brain tissue lysate (input)
Lane 2: HA723915 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA723915 in mouse brain tissue lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"