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GATA3 Recombinant Rabbit Monoclonal Antibody [PSH25-24]

Usd: 385

Specification

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Catalog# HA724518

GATA3 Recombinant Rabbit Monoclonal Antibody [PSH25-24]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

GATA3 Recombinant Rabbit Monoclonal Antibody [PSH25-24]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human GATA3 aa 1-443.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 48 kDa

Positive Control

SH-SY5Y (Human neuroblastoma cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, MCF7 (Human breast cancer cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH25-24

Reactivity Data

WB IF-Cell IHC-P
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200-1:500

Target

Function

GATA3 is a transcription factor that in humans is encoded by the GATA3 gene. Studies in animal models and humans indicate that it controls the expression of a wide range of biologically and clinically important genes. The GATA3 transcription factor is critical for the embryonic development of various tissues as well as for inflammatory and humoral immune responses and the proper functioning of the endothelium of blood vessels. GATA3 haploinsufficiency (i.e. lose of one or the two inherited GATA3 genes) results in a congenital disorder termed the Barakat syndrome. Current clinical and laboratory research is focusing on determining the benefits of directly or indirectly blocking the action of GATA3 in inflammatory and allergic diseases such as asthma. It is also proposed to be a clinically important marker for various types of cancer, particularly those of the breast. However, the role, if any, of GATA3 in the development of these cancers is under study and remains unclear.

Background References

1. Zhu Z et al. GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells. Drug Resist Updat. 2023 Jul

2. Bacha R et al. Unveiling GATA3 Signaling Pathways in Health and Disease: Mechanisms, Implications, and Therapeutic Potential. Cells. 2024 Dec

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P23771 Human

Synonyms

GATA 3 antibody

GATA binding factor 3 antibody

GATA binding protein 3 antibody

GATA-binding factor 3 antibody

Gata3 antibody

GATA3_HUMAN antibody

HDR antibody

HDRS antibody

MGC2346 antibody

MGC5199 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of GATA3 on different lysates with Rabbit anti-GATA3 antibody (<a href="/products/HA724518" style="font-weight: bold;text-decoration: underline;">HA724518</a>) at 1/5,000 dilution. <br /> <br />Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate <br />Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate <br />Lane 3: MCF7 (Human breast cancer cell) cell lysate <br />Lane 4: MDA-MB-231 (Human breast cancer cell) cell lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Low expression of GATA3 protein in MDA-MB-231 is consistent with the predicted expression pattern. <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724518" style="font-weight: bold;text-decoration: underline;">HA724518</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 48 kDa <br />Observed band size: 48 kDa

    ☑ Relative expression (RE)

    Western blot analysis of GATA3 on different lysates with Rabbit anti-GATA3 antibody (HA724518) at 1/5,000 dilution.

    Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
    Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 3: MCF7 (Human breast cancer cell) cell lysate
    Lane 4: MDA-MB-231 (Human breast cancer cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Low expression of GATA3 protein in MDA-MB-231 is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724518, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: SH-SY5Y (Human neuroblastoma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724518" style="font-weight: bold;text-decoration: underline;">HA724518</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: SH-SY5Y (Human neuroblastoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724518, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Urothelial carcinoma <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724518" style="font-weight: bold;text-decoration: underline;">HA724518</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Urothelial carcinoma
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724518, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724518" style="font-weight: bold;text-decoration: underline;">HA724518</a>, 1/200, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724518, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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