GATA4 Recombinant Rabbit Monoclonal Antibody [PSH23-98]
Catalog# HA724409
GATA4 Recombinant Rabbit Monoclonal Antibody [PSH23-98]
-
WB
-
IF-Cell
-
IHC-P
-
FC(Intra)
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
GATA4 Recombinant Rabbit Monoclonal Antibody [PSH23-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human GATA4 aa 1-442.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC(Intra)
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
Hep G2 (Human liver cancer cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, Mouse ovary tissue lysate, Rat testis tissue lysate, Rat ovary tissue lysate.
Conjugation
unconjugated
Clone Number
PSH23-98
Reactivity Data
| WB | IF-Cell | IHC-P | FC(Intra) | |
|---|---|---|---|---|
| Human |
|
|
|
|
| Mouse |
|
|
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:100
-
IHC-P
-
1:200
-
FC(Intra)
-
1:1,000
Target
Function
Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.
Background References
1. Yamada Y et al. Cardiac Reprogramming and Gata4 Overexpression Reduce Fibrosis and Improve Diastolic Dysfunction in Heart Failure With Preserved Ejection Fraction. Circulation. 2025 Feb
2. Zhou BR et al. Structural insights into the cooperative nucleosome recognition and chromatin opening by FOXA1 and GATA4. Mol Cell. 2024 Aug
Subcellular Location
Nucleus
Synonyms
ASD2 antibody
GATA 4 antibody
GATA binding protein 4 antibody
GATA-binding factor 4 antibody
gata4 antibody
GATA4_HUMAN antibody
MGC126629 antibody
Transcription factor GATA 4 antibody
Transcription factor GATA-4 antibody
Transcription factor GATA4 antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of GATA4 on different lysates with Rabbit anti-GATA4 antibody (HA724409) at 1/5,000 dilution.
Lane 1: Hep G2 (Human liver cancer cells) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblasts) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 2 minutes; ECL: K1802
HeLa is a negative control (PMID: 24696446).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724409, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 45 kDa
Observed band size: 50 kDa -
☑ Relative expression (RE)
Western blot analysis of GATA4 on different lysates with Rabbit anti-GATA4 antibody (HA724409) at 1/5,000 dilution.
Lane 1: Mouse ovary tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Skeletal muscle tissue is a negative control (PMID: 22808244).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724409, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 45 kDa
Observed band size: 50 kDa -
☑ Relative expression (RE)
Western blot analysis of GATA4 on different lysates with Rabbit anti-GATA4 antibody (HA724409) at 1/2,000 dilution.
Lane 1: Rat testis tissue lysate
Lane 2: Rat ovary tissue lysate
Lane 3: Rat skeletal muscle tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Skeletal muscle tissue is a negative control (PMID: 22808244).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724409, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 45 kDa
Observed band size: 50 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: Hep G2 (Human liver cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724409, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724409, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Skeletal muscle
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724409, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Skeletal muscle tissue is a negative control (PMID：22808244). -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724409, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Skeletal muscle
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724409, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Skeletal muscle tissue is a negative control (PMID：22808244). -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724409, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Skeletal muscle
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724409, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Skeletal muscle tissue is a negative control (PMID：22808244). -
Application: Flow Cytometry (Intra)
Species: Human
Sample: Hep G2 (Human liver cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724409 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724409 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"