GGT1 Recombinant Rabbit Monoclonal Antibody [JE38-79]
Catalog# HA722214
GGT1 Recombinant Rabbit Monoclonal Antibody [JE38-79]
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WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
GGT1 Recombinant Rabbit Monoclonal Antibody [JE38-79]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human GGT1 aa 1-100 / 569.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 61 kDa
Positive Control
HepG2 cell lysate, A549 cell lysate, human liver tissue lysate, human kidney tissue lysate, human kidney tissue.
Conjugation
unconjugated
Clone Number
JE38-79
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Gamma-glutamyltransferase 1 (GGT1), also known as CD224 (Cluster of Differentiation 224), is a human gene. Human gamma-glutamyltransferase catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. This heteroduplex enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized. This gene encodes several transcript variants; studies suggest that many transcripts of this gene family may be non-functional or represent pseudogenes. The functional transcripts which have been fully characterized have been grouped and classified as type I gamma-glutamyltransferase. Complex splicing events may take place in a tissue-specific manner, resulting in marked dissimilarity in the 5' UTRs. Several 5' UTR transcript variants of the type I gene have been identified in different tissues and cancer cells.
Background References
1. Xu G et al. GGT1 Suppresses the Development of Ferroptosis and Autophagy in Mouse Retinal Ganglion Cell Through Targeting GCLC. Eye Brain. 2023 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD224 antibody
D22S672 antibody
D22S732 antibody
Gamma glutamyl transpeptidase antibody
Gamma glutamyltransferase 1 antibody
Gamma glutamyltranspeptidase 1 antibody
Gamma-glutamyltransferase 1 antibody
Gamma-glutamyltranspeptidase 1 light chain antibody
GGT 1 antibody
GGT antibody
ExpandImages
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Western blot analysis of GGT1 on different lysates with Rabbit anti-GGT1 antibody (HA722214) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Human liver tissue lysate (40 µg/Lane)
Lane 4: Human kidney tissue lysate (40 µg/Lane)
Predicted band size: 61 kDa
Observed band size: 60-70 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722214) at 1/1,000 dilution was used in antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GGT1 antibody (HA722214) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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