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Glutathione Reductase Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1802-96

Glutathione Reductase Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Glutathione Reductase Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human Glutathione Reductase aa 273-446 / 522.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 56 kDa

Positive Control

A549 (Human lung adenocarcinoma cell) cell lysate, mouse kidney tissue lysate, mouse liver tissue lysate, rat kidney tissue lysate, rat liver tissue lysate, HCT 116 cell lysate, A549, rat epididymis tissue, human liver tissue, human thyroid gland tissue.

Conjugation

unconjugated

RRID

AB_3069196

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Glutathione plays a key role in maintaining proper function and preventing oxidative stress in human cells. It can act as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces hydrogen peroxide (H2O2), a dangerously reactive species within the cell. In addition, it plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, participates in transport, and regenerates antioxidants such and Vitamins E and C to their reactive forms. The ratio of GSSG/GSH present in the cell is a key factor in properly maintaining the oxidative balance of the cell, that is, it is critical that the cell maintains high levels of the reduced glutathione and a low level of the oxidized Glutathione disulfide. This narrow balance is maintained by glutathione reductase, which catalyzes the reduction of GSSG to GSH.

Background References

暂无

Sequence Similarity

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Subcellular Location

Cytoplasm. Mitochondrion.

UNIPROT

SwissProt: P00390 Human

SwissProt: P47791 Mouse

SwissProt: P70619 Rat

Synonyms

GLR1 antibody

GLUR antibody

Glutathione reductase antibody

Glutathione reductase mitochondrial antibody

Glutathione reductase, mitochondrial antibody

GR antibody

Gr1 antibody

GRase antibody

GRD 1 antibody

GRD1 antibody

Expand

Images

  • Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (<a href="/products/ER1802-96" style="font-weight: bold;text-decoration: underline;">ER1802-96</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate<br />Lane 2: Mouse kidney tissue lysate<br />Lane 3: Mouse liver tissue lysate<br />Lane 4: Rat kidney tissue lysate<br />Lane 5: Rat liver tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 59 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1802-96" style="font-weight: bold;text-decoration: underline;">ER1802-96</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 56 kDa<br />Observed band size: 54 kDa

    Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (ER1802-96) at 1/5,000 dilution.

    Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 2: Mouse kidney tissue lysate
    Lane 3: Mouse liver tissue lysate
    Lane 4: Rat kidney tissue lysate
    Lane 5: Rat liver tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1802-96, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 56 kDa
    Observed band size: 54 kDa

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (<a href="/products/ER1802-96" style="font-weight: bold;text-decoration: underline;">ER1802-96</a>) at 1/2,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si Glutathione Reductase cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1802-96" style="font-weight: bold;text-decoration: underline;">ER1802-96</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody (ER1802-96) at 1/2,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si Glutathione Reductase cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 54 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-96) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining GLUR in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GLUR in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLUR antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLUR antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUR antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUR antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLUR antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLUR antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Glutathione Reductase Recombinant Rabbit Monoclonal Antibody [JE31-42]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated