GPNMB Recombinant Rabbit Monoclonal Antibody [PSH0-82]
Catalog# HA721511
GPNMB Recombinant Rabbit Monoclonal Antibody [PSH0-82]
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
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HA750662
不含抗保成分
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unconjugated
Overview
Product Name
GPNMB Recombinant Rabbit Monoclonal Antibody [PSH0-82]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human GPNMB aa 1-498 / 572.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, FC
Molecular Weight
Predicted band size: 64 kDa
Positive Control
L929 cell lysate, A375 cell lysate, U-937 cell lysate, rat heart tissue lysate, B16F1 cell lysates, human lung tissue, L929.
Conjugation
unconjugated
Clone Number
PSH0-82
RRID
Reactivity Data
| WB | IHC-P | IF-Cell | FC | |
|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
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IF-Cell
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1:100
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FC
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1:500-1:1,000
Target
Function
Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. Two transcript variants encoding 560 and 572 amino acid isoforms have been characterized for this gene in humans. The mouse and rat orthologues of GPNMB are known as DC-HIL and Osteoactivin (OA), respectively. GPNMB is a type I transmembrane glycoprotein which shows homology to the pmel17 precursor, a melanocyte-specific protein. GPNMB has been reported to be expressed in various cell types, including: melanocytes, osteoclasts, osteoblasts, dendritic cells, and it is overexpressed in various cancer types. In melanocytic cells and osteoclasts the GPNMB gene is transcriptionally regulated by Microphthalmia-associated transcription factor. In osteoblast progenitor cells, Osteoactivin works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization[9] that is mediated at least in part by Bone_morphogenetic_protein_2 in a SMAD1 dependent manner to promote osteoblast differentiation. In addition, using a rat fracture model, Osteoactivin (OA) enhances the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, Osteoactivin expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.
Background References
1. Saade M et al. The Role of GPNMB in Inflammation. Front Immunol. 2021 May
2. Diaz-Ortiz ME et al. GPNMB confers risk for Parkinson\'s disease through interaction with α-synuclein. Science. 2022 Aug
Subcellular Location
Cell membrane, Melanosome membrane, Early endosome membrane.
Synonyms
Glycoprotein (transmembrane) nmb antibody
Glycoprotein nmb antibody
Glycoprotein nmb like protein antibody
Gpnmb antibody
GPNMB_HUMAN antibody
HGFIN antibody
NMB antibody
Osteoactivin antibody
Transmembrane glycoprotein antibody
Transmembrane glycoprotein HGFIN antibody
ExpandImages
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Western blot analysis of GPNMB on different lysates with Rabbit anti-GPNMB antibody (HA721511) at 1/1,000 dilution.
Lane 1: L929 cell lysate (20 µg/Lane)
Lane 2: A375 cell lysate (20 µg/Lane)
Lane 3: U-937 cell lysate (20 µg/Lane)
Lane 4: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 64 kDa
Observed band size: 80-120kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721511) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of GPNMB on B16F1 cell lysates with Rabbit anti-GPNMB antibody (HA721511) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 80-120kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721511) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-GPNMB antibody (HA721511) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721511) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of L929 cells labeling GPNMB with Rabbit anti-GPNMB antibody (HA721511) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPNMB antibody (HA721511) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of L929 cells labeling GPNMB.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721511, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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