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GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27]

Usd: 385

Specification

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Catalog# HA722217

GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human GSK3 alpha aa 50-150.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 51 kDa

Positive Control

HeLa cell lysate, A431 cell lysate, A549 cell lysate, Jurkat cell lysate, HCT 116cell lysate, HEK-293 cell lysate, U-87 MG cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, SK-Br-3 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, human ovary cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JE33-27

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Glycogen synthase kinase-3 alpha is an enzyme that in humans is encoded by the GSK3A gene. Glycogen synthase kinase 3-alpha EC 2.7.1.37 is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and various transcription factors (e.g., JUN). It also plays a role in the WNT and phosphoinositide 3-kinase (especially PIK3CG) signaling pathways.

Background References

1. Wang J et al. Quantitative phosphoproteomics reveals GSK3A substrate network is involved in the cryodamage of sperm motility. Biosci Rep. 2021 Oct

2. Zhang W et al. Active Ingredient Paeonol of Jijiu Huiyang Decoction Alleviates Isoproterenol-Induced Chronic Heart Failure via the GSK3A/PPARalpha Pathway. Oxid Med Cell Longev. 2023 Feb

Subcellular Location

Apical dendrite, axon, cytoplasm, cytosol, mitochondrion, neuronal cell body, nucleus, postsynapse, proximal dendrite.

UNIPROT

SwissProt: P49840 Human

SwissProt: Q2NL51 Mouse

SwissProt: P18265 Rat

Synonyms

DKFZp686D0638 antibody

Glycogen synthase kinase 3 alpha antibody

Glycogen synthase kinase-3 alpha antibody

GSK 3 alpha antibody

GSK 3A antibody

GSK-3 alpha antibody

Gsk3a antibody

GSK3A_HUMAN antibody

GSK3alpha antibody

Serine/threonine protein kinase GSK3A antibody

Images

  • Western blot analysis of GSK3 alpha on different lysates with Rabbit anti-GSK3 alpha antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: HCT 116cell lysate<br />Lane 6: HEK-293 cell lysate<br />Lane 7: U-87 MG cell lysate<br />Lane 8: MCF7 cell lysate<br />Lane 9: MDA-MB-231 cell lysate<br />Lane 10: SK-Br-3 cell lysate<br />Lane 11: Neuro-2a cell lysate<br />Lane 12: NIH/3T3 cell lysate<br />Lane 13: C6 cell lysate<br />Lane 14: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 51 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GSK3 alpha on different lysates with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A431 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: HCT 116cell lysate
    Lane 6: HEK-293 cell lysate
    Lane 7: U-87 MG cell lysate
    Lane 8: MCF7 cell lysate
    Lane 9: MDA-MB-231 cell lysate
    Lane 10: SK-Br-3 cell lysate
    Lane 11: Neuro-2a cell lysate
    Lane 12: NIH/3T3 cell lysate
    Lane 13: C6 cell lysate
    Lane 14: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722217) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-GSK3 alpha antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GSK3 alpha antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GSK3 alpha antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-GSK3 alpha antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722217" style="font-weight: bold;text-decoration: underline;">HA722217</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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