Gastrin Recombinant Rabbit Monoclonal Antibody [PD00-37]
Catalog# HA721159
Gastrin Recombinant Rabbit Monoclonal Antibody [PD00-37]
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
Gastrin Recombinant Rabbit Monoclonal Antibody [PD00-37]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Gastrin (internal sequence).
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 11 kDa
Positive Control
Human stomach tissue.
Conjugation
unconjugated
Clone Number
PD00-37
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
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IF-Tissue
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1:2,000
Target
Function
Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the pyloric antrum of the stomach, duodenum, and the pancreas. Gastrin binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+/H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release into the stomach cavity). Its release is stimulated by peptides in the lumen of the stomach. In the Zollinger–Ellison syndrome, gastrin is produced at excessive levels, often by a gastrinoma gastrin-producing tumor, mostly benign of the duodenum or the pancreas. In autoimmune gastritis, the immune system attacks the parietal cells leading to hypochlorhydria low stomach acid secretion. This results in an elevated gastrin level in an attempt to compensate for increased pH in the stomach. Eventually, all the parietal cells are lost and achlorhydria results leading to a loss of negative feedback on gastrin secretion. Plasma gastrin concentration is elevated in virtually all individuals with mucolipidosis type IV (mean 1507 pg/mL; range 400-4100 pg/mL) (normal 0-200 pg/mL) secondary to a constitutive achlorhydria. This finding facilitates the diagnosis of patients with this neurogenetic disorder. Additionally, elevated gastrin levels may be present in chronic gastritis resulting from H pylori infection.
Background References
1. Schubert ML. et. al. Gastric Peptides-Gastrin and Somatostatin. Compr Physiol. 2019 Dec
2. Duan S et. al. Gastrin: From Physiology to Gastrointestinal Malignancies. Function (Oxf). 2021 Nov
Subcellular Location
Secreted.
UNIPROT
Synonyms
Component II antibody
G14 antibody
G17 antibody
G34 antibody
G52 antibody
G6 antibody
GAS antibody
Gast antibody
GAST_HUMAN antibody
Gastrin antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Gastrin antibody (HA721159) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721159) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-Gastrin antibody (HA721159) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721159) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: stomach
Sample: Paraffin-embedded section
Antibody concentration: 1/2,000
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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