Gli1 Mouse Monoclonal Antibody [F8-B9]
Catalog# EM1708-22
Gli1 Mouse Monoclonal Antibody [F8-B9]
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Gli1 Mouse Monoclonal Antibody [F8-B9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P, FC
Molecular Weight
118 kDa
Positive Control
Human GLI1 recombinant protein, GLI1-hIgGFc transfected HEK293 cell lysate, Hela, HepG2, human ovarian cancer tissue, human esophageal cancer tissue.
Conjugation
unconjugated
Clone Number
F8-B9
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IF-cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:100-1:200
Target
Function
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
Background References
1. Lin Z et al. Inhibition of the CyclinD1 promoter in response to sonic hedgehog signaling pathway transduction is mediated by Gli1. Exp Ther Med 13:307-314 (2017).
2. Li P et al. Nestin Mediates Hedgehog Pathway Tumorigenesis. Cancer Res 76:5573-83 (2016).
Subcellular Location
Cytoplasm. Nucleus.
UNIPROT
Synonyms
Gli 1 antibody
GLI antibody
GLI family zinc finger 1 antibody
GLI Kruppel family member 1 antibody
gli1 antibody
GLI1_HUMAN antibody
Glioma associated oncogene 1 antibody
Glioma associated oncogene homolog 1 (zinc finger protein) antibody
Glioma associated oncogene homolog antibody
Glioma-associated oncogene antibody
ExpandImages
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ICC staining GLI1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08]
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08]
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