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Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JA31-40]

Usd: 385

Specification

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Catalog# ET1704-39

Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JA31-40]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JA31-40]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 460-509 / 515.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 59 kDa

Positive Control

A549 cell lysate, Hela, HepG2, MCF-7, human liver tissue, human stomach cancer tissue, mouse testes tissue, mouse skeletal muscle tissue.

Conjugation

unconjugated

Clone Number

JA31-40

RRID

AB_3073472

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Glucose-6-phosphate 1-dehydrogenase (G6PD) plays an important role in the pentose phosphate pathway. It is a member of the glucose-6-phosphate dehydrogenase family of proteins. G6PD is a ubiquitous enzyme that produces pentose sugars for nucleic acid synthesis, but is also involved in carbohydrate degradation, as it is one of the main producers of NADPH reducing power. G6PD has NADP as a co-factor and structural element. It can be found as a homodimer or homotetramer, and is primarily detected in lymphoblasts, granulocytes and sperm. Defects in G6PD can cause chronic non-spherocytic hemolytic anemia (CNSHA), especially in areas in which malaria is an epidemic. Individuals with a high level of G6PD-deficiency are at higher risk of acute hemolytic attacks.

Background References

1. Kaneko Y et al.Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro.Sci Rep

Sequence Similarity

Belongs to the glucose-6-phosphate dehydrogenase family.

Tissue Specificity

Isoform Long is found in lymphoblasts, granulocytes and sperm.

Post-translational Modification

Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.

Subcellular Location

Cytoplasm. Cytoplasmic side of plasma membrane. Membrane. Nucleus.

UNIPROT

SwissProt: P11413 Human

Synonyms

G6PD antibody

G6PD_HUMAN antibody

G6PD1 antibody

G6pdx antibody

Glucose 6 phosphate 1 dehydrogenase antibody

Glucose 6 phosphate dehydrogenase antibody

Glucose 6 phosphate dehydrogenase, G6PD antibody

Glucose-6-phosphate 1-dehydrogenase antibody

MET19 antibody

POS10 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Glucose 6 Phosphate Dehydrogenase on different lysates with Rabbit anti-Glucose 6 Phosphate Dehydrogenase antibody (<a href="/products/ET1704-39" style="font-weight: bold;text-decoration: underline;">ET1704-39</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si G6PD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-39" style="font-weight: bold;text-decoration: underline;">ET1704-39</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Glucose 6 Phosphate Dehydrogenase on different lysates with Rabbit anti-Glucose 6 Phosphate Dehydrogenase antibody (ET1704-39) at 1/1,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si G6PD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1,000 dilution.

    Western blot analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1,000 dilution.

  • ICC staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation