Glutamate receptor 1 Recombinant Rabbit Monoclonal Antibody [SD2010] - BSA and Azide free
Catalog# HA750281
Glutamate receptor 1 Recombinant Rabbit Monoclonal Antibody [SD2010] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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Human
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Mouse
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Rat
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ET1612-10
含抗保成分
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unconjugated
Overview
Product Name
Glutamate receptor 1 Recombinant Rabbit Monoclonal Antibody [SD2010] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Glutamate receptor 1 aa 857-906 / 906.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IHC-Fr
Molecular Weight
Predicted band size: 102 kDa
Positive Control
Human brain tissue lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
SD2010
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:500-1:1,000
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IHC-Fr
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1:200-1:500
Target
Function
Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: epsilion 1, 2, 3, 4 and one zeta subunit. The zeta subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution.
Background References
1. Chen C et al. Epigenetic modification of PKM rescues aging-related cognitive impairment. Sci Rep 6:22096 (2016).
2. Gascon E et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med 20:1444-51 (2014).
Sequence Similarity
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
Tissue Specificity
Widely expressed in brain.
Post-translational Modification
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).; Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2 (By similarity). Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation (By similarity).
Subcellular Location
Cell membrane, postsynaptic cell membrane, postsynaptic density membrane, Endoplasmic reticulum membrane, Early endosome membrane, Recycling endosome membrane, dendrite, dendritic spine.
Synonyms
GLUR 1 antibody
GLUR A antibody
AMPA 1 antibody
AMPA selective glutamate receptor 1 antibody
AMPA-selective glutamate receptor 1 antibody
GluA1 antibody
GLUH1 antibody
GluR K1 antibody
GluR-1 antibody
GluR-A antibody
ExpandImages
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Western blot analysis of Glutamate receptor 1 on different lysates with Rabbit anti-Glutamate receptor 1 antibody (HA750281) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat cerebellum tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750281) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Glutamate receptor 1 antibody (HA750281) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750281, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Glutamate receptor 1 antibody (HA750281) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750281) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Glutamate receptor 1 antibody (HA750281) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750281) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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