Glycophorin A / CD235a Recombinant Mouse Monoclonal Antibody [PSH17-08] - BSA and Azide free
Catalog# HA610368
Glycophorin A / CD235a Recombinant Mouse Monoclonal Antibody [PSH17-08] - BSA and Azide free
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FC
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Human
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unconjugated
Overview
Product Name
Glycophorin A / CD235a Recombinant Mouse Monoclonal Antibody [PSH17-08] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Species Reactivity
Human
Validated Applications
FC
Target Molecular Weight
Predicted band size: 16 kDa
Positive Control
Human red blood cells.
Conjugation
unconjugated
Clone Number
PSH17-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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FC
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1:1,000
Target
Function
Glycophorin A (MNS blood group), also known as GYPA, is a protein which in humans is encoded by the GYPA gene. GYPA has also recently been designated CD235a (cluster of differentiation 235a). Glycophorins A (GYPA; this protein) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB.
Background References
1. Kodippili GC et al. DARC, Glycophorin A, Band 3, and GLUT1 Diffusion in Erythrocytes: Insights into Membrane Complexes. Biophys J. 2020 Nov
2. Kim C et al. Induced Pluripotent Stem Cells derived CD71(+)CD235a(+) Erythroblasts Were Increased by Sirtuin 1 Activator. Int J Stem Cells. 2025 Aug
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
AI853584 antibody
Blood group--MN locus antibody
CD_antigen=CD235a antibody
CD235a antibody
GLPA_HUMAN antibody
Glycophorin A (MNS blood group) antibody
Glycophorin A antibody
Glycophorin A, included antibody
Glycophorin-A antibody
GlycophorinA antibody
ExpandImages
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Flow cytometric analysis of human red blood cells labeling Glycophorin A / CD235a.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA610368, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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