HRP Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [JM10-56]
Catalog# ET1703-12
HRP Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [JM10-56]
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WB
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Species independent
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HRP
Overview
Product Name
HRP Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [JM10-56]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide immune sequence is YPYDVPDYAC.
Species Reactivity
Species independent
Validated Applications
WB
Positive Control
C-terminal HA-tag recombinant protein.
Conjugation
HRP
Clone Number
JM10-56
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the protein of interest. The HA tag is not suitable for detection or purification of proteins from apoptotic cells since it is cleaved by Caspase-3 and / or Caspase-7 after its sequence DVPD, causing it to lose its immunoreactivity. Labeling of endogenous proteins with HA-tag using CRISPR was recently accomplished in-vivo in differentiated neurons.
Background References
1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).
2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).
Synonyms
HA epitope tag antibody
HA1 antibody
HA2 antibody
hemagglutinin antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
Images
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Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (ET1703-12) at 1/2,000 dilution.
Lane 1: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate (10 µg/Lane)
Lane 2: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate (5 µg/Lane)
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-12) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"