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FITC Conjugated Goat anti-mouse IgG polyclonal Antibody

Usd: 349

Specification

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Catalog# HA1128

FITC Conjugated Goat anti-mouse IgG polyclonal Antibody

Application

  • IF-Cell

  • IF-Tissue

  • FC

  • IHC-Fr

Reactivity

  • Mouse

Conjugation

  • FITC

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FITC Conjugated Goat anti-mouse IgG polyclonal Antibody

Antibody Type

Goat Polyclonal Antibody

Immunogen

Mouse IgG (H+L).

Product Specificity

To minimize cross-reactivity, the FITC conjugated goat anti-mouse IgG (H+L) antibodies have been highly cross-adsorbed against human IgG.

Species Reactivity

Mouse

Validated Applications

IF-Cell, IF-Tissue, FC, IHC-Fr

Conjugation

FITC

Product Features

Form

Liquid

Concentration

2 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • IF-Cell

  • 1:500-1:1,000

  • IF-Tissue

  • 1:500-1:1,000

  • FC

  • 1:1,000

  • IHC-Fr

  • 1:500-1:1,000

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium.

Background References

暂无

Images

  • Immunocytochemistry analysis of Hela cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (FITC, HA1128) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (FITC, HA1128) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of Hela cells labeling Vimentin with Mouse anti-Vimentin antibody (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>) at 1/400 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Vimentin antibody (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>) at 1/400 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (FITC, HA1128) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling Vimentin with Mouse anti-Vimentin antibody (EM0401) at 1/400 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Vimentin antibody (EM0401) at 1/400 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (FITC, HA1128) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of Jurkat cells labeling FGFR2/CD332.<br /><br />Cells were fixed and permeabilized.Then stained with the primary antibody (M1501-2, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a FITC conjugate-Goat anti-Mouse IgG Secondary antibody (HA1128) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Jurkat cells labeling FGFR2/CD332.

    Cells were fixed and permeabilized.Then stained with the primary antibody (M1501-2, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a FITC conjugate-Goat anti-Mouse IgG Secondary antibody (HA1128) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"