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Human Grancalcin/GCA, N-Twin Strep, C-His, Flag Tag Protein

Usd: 159

Specification

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Catalog# HA210563

Human Grancalcin/GCA, N-Twin Strep, C-His, Flag Tag Protein

Product Information

Product name

Human Grancalcin/GCA, N-Twin Strep, C-His, Flag Tag Protein

Catalog No.

HA210563

Predicted molecular mass

30.7 kD

Protein construction description

A DNA sequence encoding the human Grancalcin/GCA protein (P28676) (Met 1-Ile 217) was expressed with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus.

Accession

P28676

Protein construction

Twin Strep Grancalcin/GCA (1-217) His Flag

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.