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Streptomyces avidinii Streptavidin, C-His Tag Protein

Usd: 159

Specification

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Catalog# HA210570

Streptomyces avidinii Streptavidin, C-His Tag Protein

Product Information

Product name

Streptomyces avidinii Streptavidin, C-His Tag Protein

Catalog No.

HA210570

Predicted molecular mass

14.9 kD

Protein construction description

A DNA sequence encoding the Streptomyces avidinii Streptavidin protein (P22629) (Gly 38-Ser 163) was expressed with a His tag at the C-terminus.

Accession

P22629

Protein construction

Streptavidin (38-163) His

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Streptomyces avidinii

Background

Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.