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Streptomyces avidinii Streptavidin, C-His Tag Protein (HA210570)

Price: Regular price $139.00

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Catalog# HA210570

Reacts with

  • Streptomyces avidinii

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Product Details

Product Type

Protein

Product Name

Streptomyces avidinii Streptavidin, C-His Tag Protein (HA210570)

Predicted molecular mass

14.9 kD

Protein construction description

A DNA sequence encoding the Streptomyces avidinii Streptavidin protein (P22629) (Gly 38-Ser 163) was expressed with a His tag at the C-terminus.

Accession

P22629

Protein construction

Streptavidin (38-163) | His

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Streptomyces avidinii

Background

Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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