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Human PDLIM3/ALP, N-Twin Strep, C-His, Flag Tag Protein

Usd: 159

Specification

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Catalog# HA210621

Human PDLIM3/ALP, N-Twin Strep, C-His, Flag Tag Protein

This product has been cited in peer reviewed publications, see list HERE

Product Information

Product name

Human PDLIM3/ALP, N-Twin Strep, C-His, Flag Tag Protein

Catalog No.

HA210621

Predicted molecular mass

19.4 kD

Protein construction description

A DNA sequence encoding the human PDLIM3/ALP protein (Q53GG5) (Asp 111-Ser 231) was expressed with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus.

Accession

Q53GG5

Protein construction

Twin Strep PDLIM3/ALP (111-231) His Flag

Source

E.coli

Bio Activity

Testing in progress.

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy. May play a role in the organization of actin filament arrays within muscle cells.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

Citation