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Human DOT1L, N-Strep II, C-His Tag Protein

Usd: 159

Specification

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Catalog# HA210700

Human DOT1L, N-Strep II, C-His Tag Protein

Product Information

Product name

Human DOT1L, N-Strep II, C-His Tag Protein

Catalog No.

HA210700

Predicted molecular mass

43.7 kD

Protein construction description

A DNA sequence encoding the human DOT1L protein (Q8TEK3) (Met 1-Ser 351) was expressed with a Strep II tag at the N-terminus and a His tag at the C-terminus.

Accession

Q8TEK3

Protein construction

Strep II DOT1L (1-351) His

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

DOT1L (mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa (predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is involved in developmental gene transcription and maintenance of chromatin structure, and may promote mixed lineage leukemia. Within the sequence used as an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third isoform of 1739 aa has a 202 aa C-terminal extension.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.