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Wnt surrogate, Tag Free Protein

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Usd: 159

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Catalog# HA210783

Wnt surrogate, Tag Free Protein

Product Information

Product name

Wnt surrogate, Tag Free Protein

Catalog No.

HA210783

Predicted molecular mass

31.2 kD

Protein construction description

Wnt surrogate protein is comprised of the LRP-binding domain of DDK linked to a selective, high affinity binder of Frizzled receptors.

Source

HEK293

Bio Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 6-24 ng/mL.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.