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Mouse IL-12 (linked heterodimer), C-Flag Tag Protein

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Catalog# HA210785

Mouse IL-12 (linked heterodimer), C-Flag Tag Protein

Product Information

Product name

Mouse IL-12 (linked heterodimer), C-Flag Tag Protein

Catalog No.

HA210785

Predicted molecular mass

60.6 kD

Protein construction description

A DNA sequence encoding the mouse IL-12B protein (P43432) (Met 23-Ser 335) and mouse IL-12A protein (P43431) (Arg 23- Ala 215) linked by a GSGSSRGGSGSGGSGGGGSK linker was expressed with a Flag tag at the C-terminus.

Accession

P43432, P43431

Protein construction

IL-12B(23-335)-GSGSSRGGSGSGGSGGGGSK-IL-12A(23-215) Flag

Source

HEK293

Bio Activity

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 15-90 ng/mL.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Mouse

Background

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.