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Human EIF4G1, N-Twin Strep, C-His, Flag Tag Protein (HA210818)

Price: Regular price $379.00

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Catalog# HA210818

Reacts with

  • Human

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Product Details

Product Type

Protein

Product Name

Human EIF4G1, N-Twin Strep, C-His, Flag Tag Protein (HA210818)

Predicted molecular mass

35.2 kD

Protein construction description

A DNA sequence encoding the human EIF4G1 protein (Q04637-1) (Thr 330-Gln 600) was expressed with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus.

Accession

Q04637-1

Protein construction

Twin Strep | EIF4G1 (330-600) | His | Flag

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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